ArticleMolecular genetics and genomics : MGG2004
The novel mutation K87E in ribosomal protein S12 enhances protein synthesis activity during the late growth phase in Escherichia coli.
Article in Molecular genetics and genomics : MGG, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 40 citations in OpenAlex.
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- Macrophages orchestrate elimination of Shigella from the intestinal epithelial cell niche via TLR-induced IL-12 and IFN-γ.Cell host & microbe · 2025Article
- Idiosyncratic evolvability among single-point ribosomal mutants towards multi-aminoglycoside resistance.PLoS genetics · 2025Article
- Improved Natamycin Production inMicroorganisms · 2025Article
- Antibiotic resistance begets more resistance: chromosomal resistance mutations mitigate fitness costs conferred by multi-resistant clinical plasmids.Microbiology spectrum · 2024Article
- Three genes controlling streptomycin susceptibility inJournal of bacteriology · 2023Article
- Proteomic and Phenotypic Studies of Mycoplasma pneumoniae Revealed Macrolide-Resistant Mutation (A2063G) Associated Changes in Protein Composition and Pathogenicity of Type I Strains.Microbiology spectrum · 2023Article
- Comparative Genome Analysis of TwoGenes · 2022Article
- DNA Breaks-Mediated Fitness Cost Reveals RNase HI as a New Target for Selectively Eliminating Antibiotic-Resistant Bacteria.Molecular biology and evolution · 2021Article
- Genetically engineered rpsL merodiploidy impacts secondary metabolism and antibiotic resistance in Streptomyces.World journal of microbiology & biotechnology · 2021Article
- Ribosome-EngineeredApplied and environmental microbiology · 2020Article
- Genome shuffling for improving the activity of alkaline pectinase in Bacillus subtilis FS105 and its molecular mechanism.World journal of microbiology & biotechnology · 2019Article
- Combinatorial Effect of ARTP Mutagenesis and Ribosome Engineering on an Industrial Strain ofFrontiers in bioengineering and biotechnology · 2019Article
- Combined Drug Resistance Mutations Substantially Enhance Enzyme Production in Paenibacillus agaridevorans.Journal of bacteriology · 2018Article
- Insights into microbial cryptic gene activation and strain improvement: principle, application and technical aspects.The Journal of antibiotics · 2017Review
- Restrictive Streptomycin Resistance Mutations Decrease the Formation of Attaching and Effacing Lesions in Escherichia coli O157:H7 Strains.Antimicrobial agents and chemotherapy · 2013Article
- RNA-guided editing of bacterial genomes using CRISPR-Cas systems.Nature biotechnology · 2013Article
- New strategies for drug discovery: activation of silent or weakly expressed microbial gene clusters.Applied microbiology and biotechnology · 2013Review
- Identification and characterization of a novel multidrug resistance operon, mdtRP (yusOP), of Bacillus subtilis.Journal of bacteriology · 2009Article
- A novel insertion mutation in Streptomyces coelicolor ribosomal S12 protein results in paromomycin resistance and antibiotic overproduction.Antimicrobial agents and chemotherapy · 2009Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Resistance to streptomycin in bacterial cells often results from a mutation in the rpsL gene that encodes the ribosomal protein S12. We found that a particular rpsL mutation (K87E), newly identified in Escherichia coli, causes aberrant protein synthesis activity late in the growth phase. While protein synthesis decreased with age in cells in the wild-type strain, it was sustained at a high level in the mutant, as determined using living cells. This was confirmed using an in vitro protein synthesis system with poly(U) and natural mRNAs (GFP mRNA and CAT mRNA). Other classical rpsL mutations (K42N and K42T) tested did not show such an effect, indicating that this novel characteristic is typical of ribosomes bearing the K87E mutant form of S12, although the K87E mutation conferred the streptomycin resistance and error-restrictive phenotypes also seen with the K42N and K42T mutations. The K87E (but not K42N or K42T) mutant ribosomes exhibited increased stability of the 70S complex in the presence of low concentrations of magnesium. We propose that the aberrant activation of protein synthesis at the late growth phase is caused by the increased stability of the ribosome.
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