ArticleThe Journal of physiology2004
Spreading dilatation in rat mesenteric arteries associated with calcium-independent endothelial cell hyperpolarization.
Article in The Journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 93 citations in OpenAlex.
- Endothelial Cell Organization Drives Distinct Agonist-Specific CaActa physiologica (Oxford, England) · 2025Article
- Signaling and structures underpinning conducted vasodilation in human and porcine intramyocardial coronary arteries.Frontiers in cardiovascular medicine · 2022Article
- Glial Cell-Based Vascular Mechanisms and Transplantation Therapies in Brain Vessel and Neurodegenerative Diseases.Frontiers in cellular neuroscience · 2021Review
- Effects of βBMC pharmacology & toxicology · 2020Article
- Optical imaging and modulation of neurovascular responses.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2018Review
- Sustained conduction of vasomotor responses in rat mesenteric arteries in a two-compartment in vitro set-up.Acta physiologica (Oxford, England) · 2018Article
- Hyperglycaemia disrupts conducted vasodilation in the resistance vasculature of db/db mice.Vascular pharmacology · 2018Article
- KJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2017Article
- Boosting the signal: Endothelial inward rectifier KMicrocirculation (New York, N.Y. : 1994) · 2017Review
- Calcium and electrical signaling in arterial endothelial tubes: New insights into cellular physiology and cardiovascular function.Microcirculation (New York, N.Y. : 1994) · 2017Review
- Conducted dilatation to ATP and KActa physiologica (Oxford, England) · 2017Article
- Membrane potential governs calcium influx into microvascular endothelium: integral role for muscarinic receptor activation.The Journal of physiology · 2015Article
- Less is more: minimal expression of myoendothelial gap junctions optimizes cell-cell communication in virtual arterioles.The Journal of physiology · 2014Article
- Regulation of cellular communication by signaling microdomains in the blood vessel wall.Pharmacological reviews · 2014Review
- β₁-Adrenoceptor stimulation suppresses endothelial IK(Ca)-channel hyperpolarization and associated dilatation in resistance arteries.British journal of pharmacology · 2013Article
- Arachidonic acid-induced dilation in human coronary arterioles: convergence of signaling mechanisms on endothelial TRPV4-mediated Ca2+ entry.Journal of the American Heart Association · 2013Article
- Intercellular communication in the vascular wall: a modeling perspective.Microcirculation (New York, N.Y. : 1994) · 2012Review
- Calcium and electrical signalling along endothelium of the resistance vasculature.Basic & clinical pharmacology & toxicology · 2012Review
- Enhanced K(+)-channel-mediated endothelium-dependent local and conducted dilation of small mesenteric arteries from ApoE(-/-) mice.Cardiovascular research · 2011Article
- EDHF: spreading the influence of the endothelium.British journal of pharmacology · 2011Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Both ACh and levcromakalim evoke smooth muscle cell hyperpolarization and associated relaxation in rat mesenteric resistance arteries. We investigated if they could evoke conducted vasodilatation along isolated arteries, whether this reflected spreading hyperpolarization and the possible mechanism involved. Focal micropipette application of either ACh, to stimulate endothelial cell muscarinic receptors, or levcromakalim, to activate smooth muscle K(ATP) channels, each evoked a local dilatation (88 +/- 14%, n= 6 and 92 +/- 6% reversal of phenylephrine-induced tone, n= 11, respectively) that rapidly spread upstream (at 1.5 mm 46 +/- 19%, n= 6 and 57 +/- 13%, n= 9) to dilate the entire isolated artery. The local dilatation to ACh was associated with a rise in endothelial cell [Ca(2+)](i) (F/F(t = 0)= 1.22 +/- 0.33, n= 14) which did not spread beyond 0.5 mm (F/F(t = 0)= 1.01 +/- 0.01, n= 14), while the local dilatation to levcromakalim was not associated with any change in endothelial cell [Ca(2+)](i). In contrast, ACh and levcromakalim both stimulated local (12.7 +/- 1.2 mV, n= 10 and 13.5 +/- 4.7 mV, n= 10) and spreading (at 2 mm: 3.0 +/- 1.1 mV, n= 5 and 4.1 +/- 0.7 mV, n= 5) smooth muscle hyperpolarization. The spread of hyperpolarization could be prevented by cutting the artery, so was not due to a diffusible agent. Both the spreading dilatation and hyperpolarization were endothelium dependent. The injection of propidium iodide into either endothelial or smooth muscle cells revealed extensive dye coupling between the endothelial cells, but limited coupling between the smooth muscle cells. Some evidence for heterocellular spread of dye was also evident. Together, these data show that vasodilatation can spread over significant distances in mesenteric resistance arteries, and suggest this reflects an effective coupling between the endothelial cells to facilitate [Ca(2+)](i)-independent spread of hyperpolarization.
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