ArticleMolecular and cellular biology2004
Quantitative proteomic identification of six4 as the trex-binding factor in the muscle creatine kinase enhancer.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 77 citations in OpenAlex.
- Ets2 Is an Activity-Dependent Transcriptional Regulator of a Fast-Twitch Muscle Program.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- SIX transcription factors are necessary for the activation of DUX4 expression in facioscapulohumeral muscular dystrophy.Skeletal muscle · 2024Article
- Review
- The application of gene marker-assisted selection and proteomics for the best meat quality criteria and body measurements in Qinchuan cattle breed.Molecular biology reports · 2018Review
- Allele-specific quantitative proteomics unravels molecular mechanisms modulated by cis-regulatory PPARG locus variation.Nucleic acids research · 2017Article
- A quantitative proteomics approach identifies ETV6 and IKZF1 as new regulators of an ERG-driven transcriptional network.Nucleic acids research · 2016Article
- Transcriptional regulation of cranial sensory placode development.Current topics in developmental biology · 2015Review
- Chromatin enrichment for proteomics.Nature protocols · 2014Article
- Overexpression of Six1 gene suppresses proliferation and enhances expression of fast-type muscle genes in C2C12 myoblasts.Molecular and cellular biochemistry · 2013Article
- The EYA-SO/SIX complex in development and disease.Pediatric nephrology (Berlin, Germany) · 2013Review
- The Eyes Absent proteins in development and disease.Cellular and molecular life sciences : CMLS · 2013Review
- Overexpression of Six1 leads to retardation of myogenic differentiation in C2C12 myoblasts.Molecular biology reports · 2013Article
- Popular computational methods to assess multiprotein complexes derived from label-free affinity purification and mass spectrometry (AP-MS) experiments.Molecular & cellular proteomics : MCP · 2013Review
- Unbiased proteomic analysis of proteins interacting with the HIV-1 5'LTR sequence: role of the transcription factor Meis.Nucleic acids research · 2012Article
- Proteomic analysis demonstrates activator- and chromatin-specific recruitment to promoters.The Journal of biological chemistry · 2012Article
- To understand the whole, you must know the parts: unraveling the roles of protein-DNA interactions in genome regulation.The Analyst · 2011Review
- Differentiation and fiber type-specific activity of a muscle creatine kinase intronic enhancer.Skeletal muscle · 2011Article
- Sequence-specific capture of protein-DNA complexes for mass spectrometric protein identification.PloS one · 2011Article
- KLF3 regulates muscle-specific gene expression and synergizes with serum response factor on KLF binding sites.Molecular and cellular biology · 2010Article
- Revealing novel telomere proteins using in vivo cross-linking, tandem affinity purification, and label-free quantitative LC-FTICR-MS.Molecular & cellular proteomics : MCP · 2010Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Transcriptional regulatory element X (Trex) is a positive control site within the Muscle creatine kinase (MCK) enhancer. Cell culture and transgenic studies indicate that the Trex site is important for MCK expression in skeletal and cardiac muscle. After selectively enriching for the Trex-binding factor (TrexBF) using magnetic beads coupled to oligonucleotides containing either wild-type or mutant Trex sites, quantitative proteomics was used to identify TrexBF as Six4, a homeodomain transcription factor of the Six/sine oculis family, from a background of approximately 900 copurifying proteins. Using gel shift assays and Six-specific antisera, we demonstrated that Six4 is TrexBF in mouse skeletal myocytes and embryonic day 10 chick skeletal and cardiac muscle, while Six5 is the major TrexBF in adult mouse heart. In cotransfection studies, Six4 transactivates the MCK enhancer as well as muscle-specific regulatory regions of Aldolase A and Cardiac troponin C via Trex/MEF3 sites. Our results are consistent with Six4 being a key regulator of muscle gene expression in adult skeletal muscle and in developing striated muscle. The Trex/MEF3 composite sequence ([C/A]ACC[C/T]GA) allowed us to identify novel putative Six-binding sites in six other muscle genes. Our proteomics strategy will be useful for identifying transcription factors from complex mixtures using only defined DNA fragments for purification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.