ArticleMolecular and cellular biology2004
An intronic enhancer regulates splicing of the twintron of Drosophila melanogaster prospero pre-mRNA by two different spliceosomes.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- The minor spliceosome component U4atac regulates JAK/STAT signaling to modulate hematopoiesis and immune responses in Drosophila melanogaster.Nature communications · 2026Article
- A Plot Twist: When RNA Yields Unexpected Findings in Paired DNA-RNA Germline Genetic Testing.Genes · 2025Article
- Aberrant RNA splicing and therapeutic opportunities in cancers.Cancer science · 2022Review
- Therapeutic Modulation of RNA Splicing in Malignant and Non-Malignant Disease.Trends in molecular medicine · 2021Review
- Minor Intron Splicing from Basic Science to Disease.International journal of molecular sciences · 2021Review
- At the Intersection of Major and Minor Spliceosomes: Crosstalk Mechanisms and Their Impact on Gene Expression.Frontiers in genetics · 2021Review
- The carboxyl termini of RAN translated GGGGCC nucleotide repeat expansions modulate toxicity in models of ALS/FTD.Acta neuropathologica communications · 2020Article
- A functional twintron, 'zombie' twintrons and a hypermobile group II intron invading itself in plant mitochondria.Nucleic acids research · 2020Article
- New insights into minor splicing-a transcriptomic analysis of cells derived from TALS patients.RNA (New York, N.Y.) · 2019Article
- Minor intron splicing revisited: identification of new minor intron-containing genes and tissue-dependent retention and alternative splicing of minor introns.BMC genomics · 2019Article
- An Integrated Model of Minor Intron Emergence and Conservation.Frontiers in genetics · 2019Article
- Competing RNA pairings in complex alternative splicing of a 3' variable region.RNA (New York, N.Y.) · 2018Article
- Review
- midlife crisis encodes a conserved zinc-finger protein required to maintain neuronal differentiation in Drosophila.Development (Cambridge, England) · 2013Article
- Minor introns are embedded molecular switches regulated by highly unstable U6atac snRNA.eLife · 2013Article
- The half-life of the HSV-1 1.5-kb LAT intron is similar to the half-life of the 2.0-kb LAT intron.Journal of neurovirology · 2013Article
- Surprisingly high number of Twintrons in vertebrates.Biology direct · 2013Article
- U12-type spliceosomal introns of Insecta.International journal of biological sciences · 2012Article
- Article
- Regulated functional alternative splicing in Drosophila.Nucleic acids research · 2012Review
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3 authors.
Funding
Abstract
We have examined the alternative splicing of the Drosophila melanogaster prospero twintron, which contains splice sites for both the U2- and U12-type spliceosome and generates two forms of mRNA, pros-L (U2-type product) and pros-S (U12-type product). We find that twintron splicing is developmentally regulated: pros-L is abundant in early embryogenesis while pros-S displays the opposite pattern. We have established a Kc cell in vitro splicing system that accurately splices a minimal pros substrate containing the twintron and have examined the sequence requirements for pros twintron splicing. Systematic deletion and mutation analysis of intron sequences established that twintron splicing requires a 46-nucleotide purine-rich element located 32 nucleotides downstream of the U2-type 5' splice site. While this element regulates both splicing pathways, its alteration showed the severest effects on the U2-type splicing pathway. Addition of an RNA competitor containing the wild-type purine-rich element to the Kc extract abolished U2-type splicing and slightly repressed U12-type splicing, suggesting that a trans-acting factor(s) binds the enhancer element to stimulate twintron splicing. Thus, we have identified an intron region critical for prospero twintron splicing as a first step towards elucidating the molecular mechanism of splicing regulation involving competition between the two kinds of spliceosomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.