ArticleJournal of virology2004
Uncleaved NS2-3 is required for production of infectious bovine viral diarrhea virus.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed, 88 citations in OpenAlex.
- Molecular diversity and detection of pestiviruses across ruminant species in Türkiye.Tropical animal health and production · 2026Article
- AlphaFold Prediction of Protein-Protein Interactions in theInternational journal of molecular sciences · 2025Article
- Essential role ofJournal of virology · 2025Article
- Bovine viral diarrhea virus 2 strains generate deletion viral genomes primarily in the NS2 region of the viral genome.Frontiers in veterinary science · 2025Article
- Packaging defects in pestiviral NS4A can be compensated by mutations in NS2 and NS3.Journal of virology · 2023Article
- Non-structural proteins of bovine viral diarrhea virus.Virus genes · 2022Review
- Endogenous Viral Elements in Shrew Genomes Provide Insights into Pestivirus Ancient History.Molecular biology and evolution · 2022Article
- Proteolytic Processing of the Coronavirus Replicase Nonstructural Protein 14 Exonuclease Is Not Required for Virus Replication but Alters RNA Synthesis and Viral Fitness.Journal of virology · 2022Article
- DNAJC14-Independent Replication of the Atypical Porcine Pestivirus.Journal of virology · 2022Article
- Characterization of a Cytopathogenic Reporter CSFV.Viruses · 2021Article
- Membrane Topology of Pestiviral Non-Structural Protein 2 and determination of the minimal autoprotease domain.Journal of virology · 2021Article
- Deciphering the Role of Bovine Viral Diarrhea Virus Non-Structural NS4B Protein in Viral Pathogenesis.Veterinary sciences · 2020Article
- Determination of Critical Requirements for Classical Swine Fever Virus NS2-3-Independent Virion Formation.Journal of virology · 2019Article
- CRISPR/Cas9-Mediated Knockout of DNAJC14 Verifies This Chaperone as a Pivotal Host Factor for RNA Replication of Pestiviruses.Journal of virology · 2019Article
- Complex Virus-Host Interactions Involved in the Regulation of Classical Swine Fever Virus Replication: A Minireview.Viruses · 2017Review
- Molecular chaperone Jiv promotes the RNA replication of classical swine fever virus.Virus genes · 2017Article
- A positive-strand RNA virus uses alternative protein-protein interactions within a viral protease/cofactor complex to switch between RNA replication and virion morphogenesis.PLoS pathogens · 2017Article
- Chaperone-Assisted Protein Folding Is Critical for Yellow Fever Virus NS3/4A Cleavage and Replication.Journal of virology · 2016Article
- Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain).Veterinary research forum : an international quarterly journal · 2016Article
- Characterization of the Determinants of NS2-3-Independent Virion Morphogenesis of Pestiviruses.Journal of virology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Despite increasing characterization of pestivirus-encoded proteins, functions for nonstructural (NS) proteins NS2, NS2-3, NS4B, and NS5A have not yet been reported. Here we investigated the function of bovine viral diarrhea virus (BVDV) uncleaved NS2-3. To test whether NS2-3 has a discrete function, the uncleaved protein was specifically abolished in two ways: first by inserting a ubiquitin monomer between NS2 and NS3, and second by placing an internal ribosome entry site between the two proteins (a bicistronic genome). In both cases, complete processing of NS2-3 prevented infectious virion formation without affecting RNA replication. We tested the hypothesis that uncleaved NS2-3 was involved in morphogenesis by creating a bicistronic genome in which NS2-3 was restored in the second cistron. With this genome, both uncleaved NS2-3 expression and particle production returned. We then investigated the minimal regions of the polyprotein that could rescue an NS2-3 defect by developing a trans-complementation assay. We determined that the expression of NS4A in cis with NS2-3 markedly increased its activity, while p7 could be supplied in trans. Based on these data, we propose a model for NS2-3 action in virion morphogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.