ArticleGenome research2004
Numerous novel annotations of the human genome sequence supported by a 5'-end-enriched cDNA collection.
Article in Genome research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- Improving mRNA 5' coding sequence determination in the mouse genome.Mammalian genome : official journal of the International Mammalian Genome Society · 2014Article
- The TIM and TAM families of phosphatidylserine receptors mediate dengue virus entry.Cell host & microbe · 2012Article
- A Plasmodium falciparum FcB1-schizont-EST collection providing clues to schizont specific gene structure and polymorphism.BMC genomics · 2009Article
- FragIdent--automatic identification and characterisation of cDNA-fragments.BMC genomics · 2009Article
- The genome sequence of the model ascomycete fungus Podospora anserina.Genome biology · 2008Article
- Systematic analysis of mRNA 5' coding sequence incompleteness in Danio rerio: an automated EST-based approach.Biology direct · 2007Article
- Large-scale identification of novel transcripts in the human genome.Genome research · 2007Article
- The role of alternative translation start sites in the generation of human protein diversity.Molecular genetics and genomics : MGG · 2005Article
- Pilot Anopheles gambiae full-length cDNA study: sequencing and initial characterization of 35,575 clones.Genome biology · 2005Article
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A collection of 90,000 human cDNA clones generated to increase the fraction of "full-length" cDNAs available was analyzed by sequence alignment on the human genome assembly. Five hundred fifty-two gene models not found in LocusLink, with coding regions of at least 300 bp, were defined by using this collection. Exon composition proposed for novel genes showed an average of 4.7 exons per gene. In 20% of the cases, at least half of the exons predicted for new genes coincided with evolutionary conserved regions defined by sequence comparisons with the pufferfish Tetraodon nigroviridis. Among this subset, CpG islands were observed at the 5' end of 75%. In-frame stop codons upstream of the initiator ATG were present in 49% of the new genes, and 16% contained a coding region comprising at least 50% of the cDNA sequence. This cDNA resource also provided candidate small protein-coding genes, usually not included in genome annotations. In addition, analysis of a sample from this cDNA collection indicates that approximately 380 gene models described in LocusLink could be extended at their 5' end by at least one new exon. Finally, this cDNA resource provided an experimental support for annotations based exclusively on predictions, thus representing a resource substantially improving the human genome annotation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.