ArticleProceedings of the National Academy of Sciences of the United States of America1992
Quantitation of immunoglobulin mu-gamma 1 heavy chain switch region recombination by a digestion-circularization polymerase chain reaction method.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 80 citations in OpenAlex.
- CFP1 promotes B cell class switch recombination through the regulation of S region germline transcription and proliferation.iScience · 2026Article
- Differential regulation of immunoglobulin class switch recombination by the tumor suppressor p53.Scientific reports · 2026Article
- Phf5a regulates DNA repair in class switch recombination via p400 and histone H2A variant deposition.The EMBO journal · 2021Article
- Lsh/HELLS is required for B lymphocyte development and immunoglobulin class switch recombination.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Orientation-specific joining of AID-initiated DNA breaks promotes antibody class switching.Nature · 2015Article
- PP4 is essential for germinal center formation and class switch recombination in mice.PloS one · 2014Article
- Article
- YY1 controls immunoglobulin class switch recombination and nuclear activation-induced deaminase levels.Molecular and cellular biology · 2012Article
- The transcription factor BATF controls the global regulators of class-switch recombination in both B cells and T cells.Nature immunology · 2011Article
- Sense transcription through the S region is essential for immunoglobulin class switch recombination.The EMBO journal · 2011Article
- Murine gammaherpesvirus 68 LANA is essential for virus reactivation from splenocytes but not long-term carriage of viral genome.Journal of virology · 2010Article
- Switch region identity plays an important role in Ig class switch recombination.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- AID-induced decrease in topoisomerase 1 induces DNA structural alteration and DNA cleavage for class switch recombination.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Switch recombination and somatic hypermutation are controlled by the heavy chain 3' enhancer region.The Journal of experimental medicine · 2009Article
- Activation-induced deaminase-mediated class switch recombination is blocked by anti-IgM signaling in a phosphatidylinositol 3-kinase-dependent fashion.Molecular immunology · 2008Article
- AID-deficient Bcl-xL transgenic mice develop delayed atypical plasma cell tumors with unusual Ig/Myc chromosomal rearrangements.The Journal of experimental medicine · 2007Article
- Extracellular signal-regulated protein kinase 2 is required for efficient generation of B cells bearing antigen-specific immunoglobulin G.Molecular and cellular biology · 2007Article
- Expression of activation-induced cytidine deaminase is regulated by cell division, providing a mechanistic basis for division-linked class switch recombination.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Shifts in targeting of class switch recombination sites in mice that lack mu switch region tandem repeats or Msh2.The Journal of experimental medicine · 2005Article
- The 3' end of the heavy chain constant region locus enhances germline transcription and switch recombination of the four gamma genes.The Journal of experimental medicine · 2005Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
B lymphocytes expressing surface IgM with or without IgD may switch to the expression of other isotypes (IgG, IgA, or IgE) in the course of immune responses. Analyses of genomic DNA from cloned myelomas and hybridomas have shown that the isotype switch is accompanied by a rearrangement characterized by deletion of DNA between the switch (S) region of the mu gene and that associated with the new isotype, resulting in the formation of a composite S region. Measurement of this deletional rearrangement has been difficult in populations of normal B cells but would be useful for investigating the mechanism of the rearrangement and determining whether deletional rearrangement is responsible for all instances of class switching. We have developed a sensitive assay for deletional rearrangement that we designate the digestion-circularization polymerase chain reaction (PCR). In this assay, genomic DNA is digested with a restriction enzyme that recognizes sites that flank the recombined composite S region. The digested DNA is then ligated at low concentrations to favor the formation of circles. The ligation joins the 5' and 3' ends of each restriction fragment, making it possible to amplify by PCR across the ligated restriction site by using appropriate primers. From DNA that has undergone deletional rearrangement, a single-sized PCR product is produced and can be quantitated. We demonstrate here that the digestion-circularization PCR assay can detect S mu-S gamma 1 rearrangements in B cells cultured with lipopolysaccharide and interleukin 4. The assay is sensitive enough to quantitate switched cells constituting only 1-2% of the population.
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