ArticleMolecular biology of the cell1992
Inducible expression of calmodulin antisense RNA in Dictyostelium cells inhibits the completion of cytokinesis.
Article in Molecular biology of the cell, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 104 citations in OpenAlex.
- A Proteomics Analysis of Calmodulin-Binding Proteins inInternational journal of molecular sciences · 2021Article
- An EF-hand-containing Protein in Trypanosoma brucei Regulates Cytokinesis Initiation by Maintaining the Stability of the Cytokinesis Initiation Factor CIF1.The Journal of biological chemistry · 2016Article
- Calcium Signaling Is Required for Erythroid Enucleation.PloS one · 2016Article
- The cyclin-dependent kinase family in the social amoebozoan Dictyostelium discoideum.Cellular and molecular life sciences : CMLS · 2014Review
- Calmodulin protects Aurora B on the midbody to regulate the fidelity of cytokinesis.Cell cycle (Georgetown, Tex.) · 2013Article
- Separation anxiety: stress, tension and cytokinesis.Experimental cell research · 2012Review
- Calcineurin activity is required for the completion of cytokinesis.Cellular and molecular life sciences : CMLS · 2010Article
- Article
- Making the cut: the chemical biology of cytokinesis.ACS chemical biology · 2010Review
- Balance of actively generated contractile and resistive forces controls cytokinesis dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Intron analyses reveal multiple calmodulin copies in Littorina.Journal of molecular evolution · 2005Article
- The stress and strain of cytokinesis.Trends in cell biology · 2005Review
- DWWA, a novel protein containing two WW domains and an IQ motif, is required for scission of the residual cytoplasmic bridge during cytokinesis in Dictyostelium.Molecular biology of the cell · 2004Article
- Revamp a model-status and prospects of the Dictyostelium genome project.Current genetics · 2003Review
- Chaperonin 60 and mitochondrial disease in Dictyostelium.Journal of muscle research and cell motility · 2002Article
- Protein tyrosine phosphatase-PEST regulates focal adhesion disassembly, migration, and cytokinesis in fibroblasts.The Journal of cell biology · 1999Article
- The multiple roles of Cyk1p in the assembly and function of the actomyosin ring in budding yeast.Molecular biology of the cell · 1999Article
- Disruption of a dynamin homologue affects endocytosis, organelle morphology, and cytokinesis in Dictyostelium discoideum.Molecular biology of the cell · 1999Article
- Iqg1p, a yeast homologue of the mammalian IQGAPs, mediates cdc42p effects on the actin cytoskeleton.The Journal of cell biology · 1998Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The single gene encoding calmodulin in the eukaryotic microorganism Dictyostelium discoideum was cloned and sequenced. The gene was found to contain three introns, one lying immediately after the translation initiation codon. The deduced amino acid sequence indicated that Dictyostelium calmodulin contains 19 amino acid differences from vertebrate calmodulin, including extensions at both termini. Northern blot analysis showed that similar levels of calmodulin mRNA are present throughout growth and development of wild-type cells. A complete copy of the calmodulin cDNA was prepared, and an 87-base pair fragment complementary to the 5'-end of the calmodulin mRNA was subcloned into the Dictyostelium transformation vector pVEII, such that expression of the antisense transcript was driven by the discoidin I gamma promoter. Transformed cells were selected and maintained at low cell density, a condition resulting in minimal activity of the discoidin I promoter. High level expression was induced by allowing the transformants to reach high cell density or by growing them in the presence of medium conditioned by high density cells. Under these conditions, in which calmodulin mRNA and protein levels were reduced about twofold, the calmodulin antisense transformants lost the ability to complete cytokinesis. A contractile ring formed and constricted, but the midbody linking daughter cells failed to break. The resulting cell population contained multinucleated cells and networks of cells connected by cytoplasmic bridges. Normal cell division was restored when the cells were diluted to low density. These observations have identified a new point at which calmodulin may regulate cell cleavage.
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