ArticleEukaryotic cell2004
Response of the Saccharomyces cerevisiae Mpk1 mitogen-activated protein kinase pathway to increases in internal turgor pressure caused by loss of Ppz protein phosphatases.
Article in Eukaryotic cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 64 citations in OpenAlex.
- Activation of CWI pathway through high hydrostatic pressure, enhancing glycerol efflux via the aquaglyceroporin Fps1 inMolecular biology of the cell · 2023Article
- Functional mapping of the N-terminal region of the yeast moonlighting protein Sis2/Hal3 reveals crucial residues for Ppz1 regulation.The FEBS journal · 2022Article
- Fungal Hal3 (and Its Close Relative Cab3) as Moonlighting Proteins.Journal of fungi (Basel, Switzerland) · 2022Review
- The Toxic Effects of Ppz1 Overexpression Involve Nha1-Mediated Deregulation of KJournal of fungi (Basel, Switzerland) · 2021Article
- The N-Terminal Region of Yeast Protein Phosphatase Ppz1 Is a Determinant for Its Toxicity.International journal of molecular sciences · 2020Article
- Potassium starvation induces autophagy in yeast.The Journal of biological chemistry · 2020Article
- Yeast Ppz1 protein phosphatase toxicity involves the alteration of multiple cellular targets.Scientific reports · 2020Article
- Protein Phosphatase Ppz1 Is Not Regulated by a Hal3-Like Protein in Plant PathogenInternational journal of molecular sciences · 2019Article
- Integrins in disguise - mechanosensors inMicrobial cell (Graz, Austria) · 2019Review
- Mutations at the hydrophobic core affect Hal3 trimer stability, reducing its Ppz1 inhibitory capacity but not its PPCDC moonlighting function.Scientific reports · 2018Article
- PI(3,5)PMolecular biology of the cell · 2018Article
- Membrane Thickness as a Key Factor Contributing to the Activation of Osmosensors and Essential Ras Signaling Pathways.Frontiers in cell and developmental biology · 2018Review
- Shrinking Daughters: Rlm1-Dependent GGenetics · 2017Article
- Reciprocal Regulation of Target of Rapamycin Complex 1 and Potassium Accumulation.The Journal of biological chemistry · 2017Article
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- Wide-Ranging Effects of the Yeast Ptc1 Protein Phosphatase Acting Through the MAPK Kinase Mkk1.Genetics · 2016Article
- Activation of stress signalling pathways enhances tolerance of fungi to chemical fungicides and antifungal proteins.Cellular and molecular life sciences : CMLS · 2014Review
- Proteome of the nematode-trapping cells of the fungus Monacrosporium haptotylum.Applied and environmental microbiology · 2013Article
- Conserved Ser/Arg-rich motif in PPZ orthologs from fungi is important for its role in cation tolerance.The Journal of biological chemistry · 2012Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Mpk1 pathway of Saccharomyces cerevisiae is a key determinant of cell wall integrity. A genetic link between the Mpk1 kinase and the Ppz phosphatases has been reported, but the nature of this connection was unclear. Recently, the Ppz phosphatases were shown to be regulators of K+ and pH homeostasis. Here, we demonstrate that Ppz-deficient strains display increased steady-state K+ levels and sensitivity to increased KCl concentrations. Given these observations and the fact that K+ is the major determinant of intracellular turgor pressure, we reasoned that the connection between PPZ1 and -2 and MPK1 was due to the combination of increased internal turgor pressure in Ppz-deficient strains and cell wall instability observed in strains lacking MPK1. Accordingly, the MPK1 gene was up-regulated, the Mpk1 protein was overexpressed, and the phosphorylated active form was more abundant in Ppz-deficient strains. Moreover, the expression of genes previously identified as targets of the Mpk1 pathway are also up-regulated in strains lacking PPZ1 and -2. The transcriptional and posttranslational modifications of Mpk1 were not observed when the internal K+ concentration (and thus turgor pressure) was lowered by disrupting the TRK1 and -2 K+ transporter genes or when the cell wall was stabilized by the addition of sorbitol. Moreover, we present genetic evidence showing that both the Wsc1 and Mid2 branches of the Mpk1 pathway contribute to this response. Finally, despite its role in G1/S transition, increased levels of activated Mpk1 do not appear to be responsible for the cell cycle phenotype observed in Ppz-deficient strains.
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