ArticleProceedings of the National Academy of Sciences of the United States of America2004
Whole-genome shotgun assembly and comparison of human genome assemblies.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed.
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- Complete genome sequencing of Bacillus sp. TK-2, analysis of its cold evolution adaptability.Scientific reports · 2021Article
- Complete genome of a unicellular parasite (Microbial genomics · 2020Article
- HUPAN: a pan-genome analysis pipeline for human genomes.Genome biology · 2019Article
- Genome sequencing-the dawn of a game-changing era.Heredity · 2019Review
- Analysis of the Aedes albopictus C6/36 genome provides insight into cell line utility for viral propagation.GigaScience · 2018Article
- GenomeLandscaper: Landscape analysis of genome-fingerprints maps assessing chromosome architecture.Scientific reports · 2018Article
- UsingBioMed research international · 2018Review
- A draft genome sequence for theF1000Research · 2018Article
- Dissecting evolution and disease using comparative vertebrate genomics.Nature reviews. Genetics · 2017Review
- Sharing Data to Build a Medical Information Commons: From Bermuda to the Global Alliance.Annual review of genomics and human genetics · 2017Review
- Hybrid assembly with long and short reads improves discovery of gene family expansions.BMC genomics · 2017Article
18 more citing papers are in PubMed but not listed here.
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36 authors.
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No grant is acknowledged in the PubMed record.
Abstract
We report a whole-genome shotgun assembly (called WGSA) of the human genome generated at Celera in 2001. The Celera-generated shotgun data set consisted of 27 million sequencing reads organized in pairs by virtue of end-sequencing 2-kbp, 10-kbp, and 50-kbp inserts from shotgun clone libraries. The quality-trimmed reads covered the genome 5.3 times, and the inserts from which pairs of reads were obtained covered the genome 39 times. With the nearly complete human DNA sequence [National Center for Biotechnology Information (NCBI) Build 34] now available, it is possible to directly assess the quality, accuracy, and completeness of WGSA and of the first reconstructions of the human genome reported in two landmark papers in February 2001 [Venter, J. C., Adams, M. D., Myers, E. W., Li, P. W., Mural, R. J., Sutton, G. G., Smith, H. O., Yandell, M., Evans, C. A., Holt, R. A., et al. (2001) Science 291, 1304-1351; International Human Genome Sequencing Consortium (2001) Nature 409, 860-921]. The analysis of WGSA shows 97% order and orientation agreement with NCBI Build 34, where most of the 3% of sequence out of order is due to scaffold placement problems as opposed to assembly errors within the scaffolds themselves. In addition, WGSA fills some of the remaining gaps in NCBI Build 34. The early genome sequences all covered about the same amount of the genome, but they did so in different ways. The Celera results provide more order and orientation, and the consortium sequence provides better coverage of exact and nearly exact repeats.
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