ArticleProceedings of the National Academy of Sciences of the United States of America2004
Propagation of infectious human papillomavirus type 16 by using an adenovirus and Cre/LoxP mechanism.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 56 citations in OpenAlex.
- Generation of a spontaneous murine HPV + oral cancer model with site-specific oncogene insertion using CRISPR-SONIC.Cell & bioscience · 2025Article
- Experimental Support for Human Papillomavirus Genome Amplification Early after Infectious Delivery.Journal of virology · 2023Article
- Human Papillomavirus Genome Copy Number Is Maintained by S-Phase Amplification, Genome Loss to the Cytosol during Mitosis, and Degradation in GJournal of virology · 2023Article
- Organoids in recapitulating tumorigenesis driven by risk factors: Current trends and future perspectives.International journal of biological sciences · 2022Review
- Genome-Wide Transcriptome Analysis of Human Papillomavirus 16-Infected Primary Keratinocytes Reveals Subtle Perturbations Mostly due to E7 Protein Expression.Journal of virology · 2020Article
- The Role of Promyelocytic Leukemia Nuclear Bodies During HPV Infection.Frontiers in cellular and infection microbiology · 2020Review
- Innervation of cervical carcinoma is mediated by cancer-derived exosomes.Gynecologic oncology · 2019Article
- Modelling human papillomavirus biology in oropharyngeal keratinocytes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- An epithelial organoid model with Langerhans cells for assessing virus-host interactions.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
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- The Known and Potential Intersections of Rab-GTPases in Human Papillomavirus Infections.Frontiers in cell and developmental biology · 2019Review
- Induction of Interferon Kappa in Human Papillomavirus 16 Infection by Transforming Growth Factor Beta-Induced Promoter Demethylation.Journal of virology · 2018Article
- A new cell culture model to genetically dissect the complete human papillomavirus life cycle.PLoS pathogens · 2018Article
- An oral keratinocyte life cycle model identifies novel host genome regulation by human papillomavirus 16 relevant to HPV positive head and neck cancer.Oncotarget · 2017Article
- Incoming human papillomavirus 16 genome is lost in PML protein-deficient HaCaT keratinocytes.Cellular microbiology · 2017Article
- Incoming human papillomavirus type 16 genome resides in a vesicular compartment throughout mitosis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Activation of NF-κB by human papillomavirus 16 E1 limits E1-dependent viral replication through degradation of E1.Journal of virology · 2015Article
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- Identification and validation of reference genes for expression studies in human keratinocyte cell lines treated with and without interferon-γ - a method for qRT-PCR reference gene determination.Experimental dermatology · 2012Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Human papillomavirus type 16 (HPV16) infection is a major risk factor for the development of squamous cell cancers of the cervix and of the head and neck. A major barrier to understanding the progression from initial infection to cancer has been the lack of in vitro models that allow infection, replication, and persistence of the viral genome as an episome in differentiated epithelial cells. To overcome this barrier, we designed an adenoviral delivery vector that contained a full HPV16 genome flanked by LoxP homologous recombination sites and a fluorescent reporter that was expressed only after the HPV genome was excised by Cre recombinase. This system delivered circular HPV16 genomes to cervical epithelial cells and well differentiated human airway epithelia. After delivery, the HPV16 genome replicated and persisted as an episome in cervical keratinocytes. These cells developed an immortalized phenotype and a dysplastic epithelial appearance. Moreover, induction of differentiation led to the expression of late genes and production of infectious HPV16 virions. This work provides a means of introducing biologically active HPV genomes into epithelial cells, which are normally difficult to transfect. These methods allow the study of HPV genome replication and gene expression in the earliest stages of HPV genome establishment, and they may provide a means to study nononcogenic HPV viral types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.