ArticlePlanta2004
Mutation of the regulatory phosphorylation site of tobacco nitrate reductase results in high nitrite excretion and NO emission from leaf and root tissue.
Article in Planta, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 113 citations in OpenAlex.
- Inoculation ofApplied and environmental microbiology · 2026Article
- Functional divergence and symbiotic significance of nitrate reductase isoforms in Medicago truncatula.Plant physiology · 2026Article
- Effects of Fe and Zn alone and combined treatment on Triticum aestivum L. seed germination.BMC plant biology · 2025Article
- Multiple Ways of Nitric Oxide Production in Plants and Its Functional Activity under Abiotic Stress Conditions.International journal of molecular sciences · 2023Review
- Vacuolar nitrate efflux requires multiple functional redundant nitrate transporter inFrontiers in plant science · 2022Article
- Constitutive activation of nitrate reductase in tobacco alters flowering time and plant biomass.Scientific reports · 2021Article
- The influence of altered sink-source balance on the plant growth and yield of greenhouse tomato.Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Nitrogen Depletion Blocks Growth Stimulation Driven by the Expression of Nitric Oxide Synthase in Tobacco.Frontiers in plant science · 2020Article
- Hemoglobin as a probe for estimation of nitric oxide emission from plant tissues.Plant methods · 2019Article
- Signal Dynamics and Interactions during Flooding Stress.Plant physiology · 2018Review
- Putative Connections Between Nitrate ReductaseFrontiers in plant science · 2018Article
- Tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis.Scientific reports · 2017Article
- Overexpression of E3 Ubiquitin Ligase GeneFrontiers in plant science · 2017Article
- Nitrate Assimilation inFrontiers in microbiology · 2017Article
- Nitric Oxide, Ethylene, and Auxin Cross Talk Mediates Greening and Plastid Development in Deetiolating Tomato Seedlings.Plant physiology · 2016Article
- Nitrite reduction by molybdoenzymes: a new class of nitric oxide-forming nitrite reductases.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2015Review
- The nitric oxide production in the moss Physcomitrella patens is mediated by nitrate reductase.PloS one · 2015Article
- Molecular components of nitrate and nitrite efflux in yeast.Eukaryotic cell · 2014Article
- Current status and proposed roles for nitric oxide as a key mediator of the effects of extracellular nucleotides on plant growth.Frontiers in plant science · 2013Review
- In silico characterization of a nitrate reductase gene family and analysis of the predicted proteins from the moss Physcomitrella patens.Communicative & integrative biology · 2012Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In wild-type Nicotiana plumbaginifolia Viv. and other higher plants, nitrate reductase (NR) is regulated at the post-translational level and is rapidly inactivated in response to, for example, a light-to-dark transition. This inactivation is caused by phosphorylation of a conserved regulatory serine residue, Ser 521 in tobacco, and interaction with divalent cations or polyamines, and 14-3-3 proteins. The physiological importance of the post-translational NR modulation is presently under investigation using a transgenic N. plumbaginifolia line. This line expresses a mutated tobacco NR where Ser 521 has been changed into aspartic acid (Asp) by site-directed mutagenesis, resulting in a permanently active NR enzyme. When cut leaves or roots of this line (S(521)) were placed in darkness in a buffer containing 50 mM KNO(3), nitrite was excreted from the tissue at rates of 0.08-0.2 micromol (g FW)(-1) h(-1) for at least 5 h. For the control transgenic plant (C1), which had the regulatory serine of NR intact, nitrite excretion was low and halted completely after 1-3 h. Without nitrate in the buffer in which the tissue was immersed, nitrite excretion was also low for S(521), although 20-40 micromol (g FW)(-1) nitrate was present inside the tissue. Apparently, stored nitrate was not readily available for reduction in darkness. Leaf tissue and root segments of S(521) also emitted much more nitric oxide (NO) than the control. Importantly, NO emission from leaf tissue of S(521) was higher in the dark than in the light, opposite to what was usually observed when post-translational NR modulation was operating.
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