ArticleNature cell biology2004
Non-proteolytic inactivation of geminin requires CDK-dependent ubiquitination.
Article in Nature cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 73 citations in OpenAlex.
- Geminin inhibits DNA replication licensing by sterically blocking CDT1-MCM2 interactions.Nature communications · 2025Article
- ThemBio · 2017Article
- Behavior of replication origins in Eukaryota - spatio-temporal dynamics of licensing and firing.Cell cycle (Georgetown, Tex.) · 2015Review
- Deregulated origin licensing leads to chromosomal breaks by rereplication of a gapped DNA template.Genes & development · 2013Article
- Cell cycle-dependent subcellular translocation of the human DNA licensing inhibitor geminin.The Journal of biological chemistry · 2013Article
- The spindle checkpoint, APC/C(Cdc20), and APC/C(Cdh1) play distinct roles in connecting mitosis to S phase.The Journal of cell biology · 2013Article
- Aurora-A controls pre-replicative complex assembly and DNA replication by stabilizing geminin in mitosis.Nature communications · 2013Article
- Dynamic association of ORCA with prereplicative complex components regulates DNA replication initiation.Molecular and cellular biology · 2012Article
- Preparation and use of Xenopus egg extracts to study DNA replication and chromatin associated proteins.Methods (San Diego, Calif.) · 2012Article
- Re-replication induced by geminin depletion occurs from G2 and is enhanced by checkpoint activation.Journal of cell science · 2012Article
- Distinct activities of the anaphase-promoting complex/cyclosome (APC/C) in mouse embryonic cells.Cell cycle (Georgetown, Tex.) · 2012Article
- The dynamics of replication licensing in live Caenorhabditis elegans embryos.The Journal of cell biology · 2012Article
- Dynamic interactions of high Cdt1 and geminin levels regulate S phase in early Xenopus embryos.Development (Cambridge, England) · 2012Article
- DNA replication licensing control and rereplication prevention.Protein & cell · 2010Review
- Quaternary structure of the human Cdt1-Geminin complex regulates DNA replication licensing.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Structure of the Cdt1 C-terminal domain: conservation of the winged helix fold in replication licensing factors.Protein science : a publication of the Protein Society · 2009Article
- MAP kinase dependent cyclinE/cdk2 activity promotes DNA replication in early sea urchin embryos.Developmental biology · 2009Article
- Geminin stabilizes Cdt1 during meiosis in Xenopus oocytes.The Journal of biological chemistry · 2009Article
- NF-kappaB and Snail1a coordinate the cell cycle with gastrulation.The Journal of cell biology · 2009Article
- Repression of nascent strand elongation by deregulated Cdt1 during DNA replication in Xenopus egg extracts.Molecular biology of the cell · 2009Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
In late mitosis and G1, a complex of the essential initiation proteins Mcm2-7 are assembled onto replication origins to 'license' them for initiation. At other times licensing is inhibited by cyclin-dependent kinases (CDKs) and geminin, thus ensuring that origins fire only once per cell cycle. Here we show that, paradoxically, CDKs are also required to inactivate geminin and activate the licensing system. On exit from metaphase in Xenopus laevis egg extracts, CDK-dependent activation of the anaphase-promoting complex (APC/C) results in the transient polyubiquitination of geminin. This ubiquitination triggers geminin inactivation without requiring ubiquitin-dependent proteolysis, and is essential for replication origins to become licensed. This reveals an unexpected role for CDKs and ubiquitination in activating chromosomal DNA replication.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.