ArticleMolecular biology of the cell2004
Rapid, endoplasmic reticulum-independent diffusion of the mitotic Golgi haze.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 76 citations in OpenAlex.
- Structural Organization and Function of the Golgi Ribbon During Cell Division.Frontiers in cell and developmental biology · 2022Article
- Unscrambling exit site patterns on the endoplasmic reticulum as a quenched demixing process.Biophysical journal · 2021Article
- PML Bodies in Mitosis.Cells · 2019Review
- Sphingolipids and lipid rafts: Novel concepts and methods of analysis.Chemistry and physics of lipids · 2018Review
- Precise and economic FIB/SEM for CLEM: with 2 nm voxels through mitosis.Histochemistry and cell biology · 2018Article
- PLCζ Induced CaFrontiers in cell and developmental biology · 2018Article
- Inducible Inhibition of Gβγ Reveals Localization-dependent Functions at the Plasma Membrane and Golgi.The Journal of biological chemistry · 2017Article
- Golgi enzymes do not cycle through the endoplasmic reticulum during protein secretion or mitosis.Molecular biology of the cell · 2017Article
- ER trapping reveals Golgi enzymes continually revisit the ER through a recycling pathway that controls Golgi organization.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Mechanisms and Regulation of the Mitotic Inheritance of the Golgi Complex.Frontiers in cell and developmental biology · 2015Review
- Multifunctional roles for the protein translocation machinery in RNA anchoring to the endoplasmic reticulum.The Journal of biological chemistry · 2014Article
- MEK1 inactivates Myt1 to regulate Golgi membrane fragmentation and mitotic entry in mammalian cells.The EMBO journal · 2013Article
- Cholesterol dependence of collagen and echovirus 1 trafficking along the novel α2β1 integrin internalization pathway.PloS one · 2013Article
- Phosphoregulation of STIM1 leads to exclusion of the endoplasmic reticulum from the mitotic spindle.Current biology : CB · 2012Article
- Modular organization of the mammalian Golgi apparatus.Current opinion in cell biology · 2012Review
- cis-Golgi proteins accumulate near the ER exit sites and act as the scaffold for Golgi regeneration after brefeldin A treatment in tobacco BY-2 cells.Molecular biology of the cell · 2012Article
- Endocytosis and signaling: cell logistics shape the eukaryotic cell plan.Physiological reviews · 2012Review
- Golgi biogenesis.Cold Spring Harbor perspectives in biology · 2011Review
- PERK (EIF2AK3) regulates proinsulin trafficking and quality control in the secretory pathway.Diabetes · 2010Article
- Influenza virus activates inflammasomes via its intracellular M2 ion channel.Nature immunology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Early in mitosis, the mammalian Golgi apparatus disassembles, and fluorescence microscopy reveals Golgi clusters and an extensive, nonresolvable haze that either represents scattered vesicles or a merged endoplasmic reticulum (ER)-Golgi compartment. To help decide between these alternatives, we have carried out a combined microscopic and pharmacological analysis, by using a BS-C-1 cell line stably coexpressing ER and Golgi markers. Video fluorescence microscopy showed that these two organelles were morphologically distinguishable at all stages of mitosis, and photobleaching experiments showed that diffusion of the Golgi marker was unaffected by the presence of the ER. Fragmentation of the ER by using filipin III completely blocked diffusion of the ER marker but had no effect on the Golgi marker, unless it was first relocated to the ER by using brefeldin A. The Golgi haze was also studied using BODIPY ceramide. Its diffusion was slower in mitotic Golgi than in mitotic ER, but similar to that of a Golgi enzyme marker in the mitotic Golgi haze or in Golgi vesicles generated by ilimaquinone. Together, these results support the idea that the Golgi and the ER remain separate during mitosis and strongly suggest that Golgi markers move by vesicle diffusion, as opposed to lateral diffusion in continuous membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.