ArticleMolecular biology of the cell2004
Tumor suppressor activity of profilin requires a functional actin binding site.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 90 citations in OpenAlex.
- Profilin and Its Isoforms: Unravelling the Functional Diversity Across Life Forms.Current protein & peptide science · 2026Review
- Actin-binding protein profilin1 is an important determinant of cellular phosphoinositide control.The Journal of biological chemistry · 2024Article
- Proteomic Alteration in the Progression of Multiple Myeloma: A Comprehensive Review.Diagnostics (Basel, Switzerland) · 2023Review
- Profilin-1 regulates DNA replication forks in a context-dependent fashion by interacting with SNF2H and BOD1L.Nature communications · 2022Article
- Biochemical characterization of actin assembly mechanisms with ALS-associated profilin variants.European journal of cell biology · 2022Article
- Cancer-associated exportin-6 upregulation inhibits the transcriptionally repressive and anticancer effects of nuclear profilin-1.Cell reports · 2021Article
- SerFrontiers in cell and developmental biology · 2021Article
- Profilin: many facets of a small protein.Biophysical reviews · 2020Review
- Neisseria meningitidis Induces Pathology-Associated Cellular and Molecular Changes in Trigeminal Schwann Cells.Infection and immunity · 2020Article
- Profilin choreographs actin and microtubules in cells and cancer.International review of cell and molecular biology · 2020Article
- PFN1 and integrin-β1/mTOR axis involvement in cornea differentiation of fibroblast limbal stem cells.Journal of cellular and molecular medicine · 2019Article
- A loss of profilin-1 in late-stage oral squamous cell carcinoma.Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology · 2017Article
- A balanced level of profilin-1 promotes stemness and tumor-initiating potential of breast cancer cells.Cell cycle (Georgetown, Tex.) · 2017Article
- ACTIN-DIRECTED TOXIN. ACD toxin-produced actin oligomers poison formin-controlled actin polymerization.Science (New York, N.Y.) · 2015Article
- Subcellular localization and Ser-137 phosphorylation regulate tumor-suppressive activity of profilin-1.The Journal of biological chemistry · 2015Article
- Silencing profilin-1 inhibits gastric cancer progression via integrin β1/focal adhesion kinase pathway modulation.World journal of gastroenterology · 2015Article
- Profilin1 regulates invadopodium maturation in human breast cancer cells.European journal of cell biology · 2015Article
- Epithelial morphological reversion drives Profilin-1-induced elevation of p27(kip1) in mesenchymal triple-negative human breast cancer cells through AMP-activated protein kinase activation.Cell cycle (Georgetown, Tex.) · 2015Article
- Identification of proteins associated with lymph node metastasis of gastric cancer.Journal of cancer research and clinical oncology · 2014Article
- A high-content, multiplexed screen in human breast cancer cells identifies profilin-1 inducers with anti-migratory activities.PloS one · 2014Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Profilin 1 (PFN1) is a regulator of the microfilament system and is involved in various signaling pathways. It interacts with many cytoplasmic and nuclear ligands. The importance of PFN1 for human tissue differentiation has been demonstrated by the findings that human cancer cells, expressing conspicuously low PFN1 levels, adopt a nontumorigenic phenotype upon raising their PFN1 level. In the present study, we characterize the ligand binding site crucial for profilin's tumor suppressor activity. Starting with CAL51, a human breast cancer cell line highly tumorigenic in nude mice, we established stable clones that express PFN1 mutants differentially defective in ligand binding. Clones expressing PFN1 mutants with reduced binding to either poly-proline-stretch ligands or phosphatidyl-inositol-4,5-bisphosphate, but with a functional actin binding site, were normal in growth, adhesion, and anchorage dependence, with only a weak tendency to elicit tumors in nude mice, similar to controls expressing wild-type PFN1. In contrast, clones expressing a mutant with severely reduced capacity to bind actin still behaved like the parental CAL51 and were highly tumorigenic. We conclude that the actin binding site on profilin is instrumental for normal differentiation of human epithelia and the tumor suppressor function of PFN1.
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