ArticleProceedings of the National Academy of Sciences of the United States of America2004
Association of rat8 with Fyn protein kinase via lipid rafts is required for rat mammary cell differentiation in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
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- The Interferon-Stimulated Gene Ifitm3 Restricts West Nile Virus Infection and Pathogenesis.Journal of virology · 2016Article
- DMSO Enhances TGF-β Activity by Recruiting the Type II TGF-β Receptor From Intracellular Vesicles to the Plasma Membrane.Journal of cellular biochemistry · 2016Article
- Ethanol Enhances TGF-β Activity by Recruiting TGF-β Receptors From Intracellular Vesicles/Lipid Rafts/Caveolae to Non-Lipid Raft Microdomains.Journal of cellular biochemistry · 2016Article
- IFITM-Family Proteins: The Cell's First Line of Antiviral Defense.Annual review of virology · 2014Article
- HIV-1 mutates to evade IFITM1 restriction.Virology · 2014Article
- Lyn, a Src family kinase, regulates activation of epidermal growth factor receptors in lung adenocarcinoma cells.Molecular cancer · 2013Article
- The N-terminal region of IFITM3 modulates its antiviral activity by regulating IFITM3 cellular localization.Journal of virology · 2012Article
- Characterization of the osteoblast-specific transmembrane protein IFITM5 and analysis of IFITM5-deficient mice.Journal of bone and mineral metabolism · 2011Article
- Distinct populations of tumor-initiating cells derived from a tumor generated by rat mammary cancer stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The properties of a mammary gland cancer stem cell.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Expression of the mouse fragilis gene products in immune cells and association with receptor signaling complexes.Genes and immunity · 2006Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously identified rat8 in the pathway involved in epithelial cell differentiation that occurs in the rat mammary gland at pregnancy when tubules and alveoli are formed. rat8, which encodes an IFN-inducible membrane protein, is the rat homologue of the mouse gene fragilis. By differential detergent extraction and isopycnic sucrose density gradients, we show that rat8 protein is associated to lipid membrane domains together with Lyn and Fyn, members of the Src tyrosine kinase family. We also show that recruitment of rat8 to lipid membrane domains is a necessary step in mammary epithelial cell differentiation. Immunoprecipitation analysis, performed with an anti-Fyn protein antibody, shows that rat8 was present in the Fyn immunoprecipitate. Antisense oligonucleotides, used to inhibit Fyn protein expression, block mammary cell differentiation. Taken together, these results suggest that the functional interaction, via lipid membrane domains, of rat8 and Fyn proteins is required for mammary cell differentiation. Therefore, rat8, like fragilis, may be involved in developmental decisions and the demarcation of a subset of cells in the mammary gland that cause epithelial cells to develop into a network of tubuloalveolar structures involved in secretion.
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