ArticleThe Journal of physiology2004
Modulation of spontaneous Ca2+-activated Cl- currents in the rabbit corpus cavernosum by the nitric oxide-cGMP pathway.
Article in The Journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Evaluation of electrophysiological changes after radical prostatectomy and their relationship with erectile function recovery.International journal of impotence research · 2025Article
- Electrophysiological and biophysical perspectives on the clitoral corpus cavernosum and its role in female sexual arousal disorder.Frontiers in physiology · 2025Review
- CaPhysiological reports · 2022Article
- Oscillating calcium signals in smooth muscle cells underlie the persistent basal tone of internal anal sphincter.Journal of cellular physiology · 2021Article
- Role of mucosa in generating spontaneous activity in the guinea pig seminal vesicle.The Journal of physiology · 2017Article
- Basally activated nonselective cation currents regulate the resting membrane potential in human and monkey colonic smooth muscle.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- Functions of ICC-like cells in the urinary tract and male genital organs.Journal of cellular and molecular medicine · 2010Review
- Smooth muscle cell calcium activation mechanisms.The Journal of physiology · 2008Review
- Membrane current oscillations in descending vasa recta pericytes.American journal of physiology. Renal physiology · 2008Article
- Regional differences in nitrergic innervation of the smooth muscle of murine lower oesophageal sphincter.British journal of pharmacology · 2008Article
- Interaction between interstitial cells and smooth muscles in the lower urinary tract and penis.The Journal of physiology · 2006Review
- Activation of the cGMP/PKG pathway inhibits electrical activity in rabbit urethral interstitial cells of Cajal by reducing the spatial spread of Ca2+ waves.The Journal of physiology · 2006Article
- Interaction between spontaneous and neurally mediated regulation of smooth muscle tone in the rabbit corpus cavernosum.The Journal of physiology · 2005Article
- Possibility of inhibition of calcium-activated chloride channel rescuing erectile failures in diabetes.International journal of impotence researchArticle
- A survey of commonalities relevant to function and dysfunction in pelvic and sexual organs.International journal of impotence researchReview
- Ultrastructural features and possible functional role of kit-positive interstitial cells in the guinea pig corpus cavernosum.International journal of impotence researchArticle
- The promise of inhibition of smooth muscle tone as a treatment for erectile dysfunction: where are we now?International journal of impotence researchReview
- Calcium-activated chloride channels in the corpus cavernosum: recent developments and future of a key cellular component of the erectile process.International journal of impotence researchReview
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The whole-cell perforated patch clamp technique was used to study membrane currents in isolated rabbit corpus cavernosum smooth muscle cells. Depolarization from -80 mV to the range -40 to -10 mV evoked a nifedipine-sensitive Ca(2+) current that was followed by a slower inward current that activated over several hundred milliseconds. The slow current reversed near the Cl(-) equilibrium potential (E(Cl)) and was reduced by anthracene-9-carboxylic acid (A9C; 1 mm) and niflumic acid (100 microm), suggesting that it was a Ca(2+)-activated Cl(-) current. When held constantly at -60 mV, over 70% of cells fired spontaneous transient inward currents (STICs), the amplitudes of which were reduced by A9C and niflumic acid. STICs reversed near E(Cl) in a symmetrical Cl(-) gradient and when [Cl(-)](o) was substituted with glutamate or I(-), the reversal potential shifted to more positive or more negative values, respectively, confirming that STICs were mediated by Cl(-) channels. STICS were also blocked by cyclopiazonic acid, 2-aminoethoxydiphenyl borate (2-APB) and 2-nitro-4-carboxyl-N,N-diphenylcarbamate (NCDC), suggesting that they depended on IP(3)-mediated Ca(2+)-release from the sarcoplasmic reticulum. Modulation by the NO-cGMP pathway was investigated by applying nitrosocysteine, 3-(5-hydroxymethyl-2-furyl)-1-benzyl indazole (YC-1), and 8-bromo cGMP, all three of which abolished STIC activity. YC-1 also reduced noradrenaline-evoked inward currents, but had no effect on similar currents evoked by caffeine, suggesting that cGMP selectively inhibited IP(3)-mediated Ca(2+) release. We propose that Ca(2+)-activated Cl(-) currents underlie detumescent tone in the corpus cavernosum, and that modulation of this mechanism by the NO-cGMP pathway is important during penile erection.
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