ArticleJournal of clinical microbiology2004
PCR assays for identification of Coccidioides posadasii based on the nucleotide sequence of the antigen 2/proline-rich antigen.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed.
- Diagnosis of Human Endemic Mycoses Caused by Thermally Dimorphic Fungi: From Classical to Molecular Methods.Journal of fungi (Basel, Switzerland) · 2024Review
- Molecular Diagnostics for Invasive Fungal Diseases: Current and Future Approaches.Journal of fungi (Basel, Switzerland) · 2024Review
- Clinical Cases of Coccidioidomycosis in the Americas in the Period 1950-2021: Epidemiology Data, Diagnosis, and Treatment.Life (Basel, Switzerland) · 2023Review
- Coccidioidomycosis Osteoarticular Dissemination.Journal of fungi (Basel, Switzerland) · 2023Review
- Molecular Diagnosis of Endemic Mycoses.Journal of fungi (Basel, Switzerland) · 2022Review
- Real-time PCR assay for detection and differentiation of Coccidioides immitis and Coccidioides posadasii from culture and clinical specimens.PLoS neglected tropical diseases · 2021Article
- Recognition of Diagnostic Gaps for Laboratory Diagnosis of Fungal Diseases: Expert Opinion from the Fungal Diagnostics Laboratories Consortium (FDLC).Journal of clinical microbiology · 2021Article
- Coccidioidomycosis in Brazil: Historical Challenges of a Neglected Disease.Journal of fungi (Basel, Switzerland) · 2021Review
- Primary Cutaneous Coccidioidomycosis: An Update.American journal of clinical dermatology · 2020Review
- Coccidioidomycosis: Epidemiology, Fungal Pathogenesis, and Therapeutic Development.Current tropical medicine reports · 2019Article
- Update on the Epidemiology of coccidioidomycosis in the United States.Medical mycology · 2019Review
- Selection of Specific Peptides forMolecules (Basel, Switzerland) · 2018Article
- Development of a real-time PCR Assay for identification of Coccidioides immitis by use of the BD Max system.Journal of clinical microbiology · 2015Article
- AFLP analysis reveals high genetic diversity but low population structure in Coccidioides posadasii isolates from Mexico and Argentina.BMC infectious diseases · 2013Article
- Antigens of Coccidioides posadasii as an important tool for the immunodiagnosis of coccidioidomycosis.Mycopathologia · 2013Article
- Histopathologic diagnosis of fungal infections in the 21st century.Clinical microbiology reviews · 2011Review
- The utility of Coccidioides polymerase chain reaction testing in the clinical setting.Mycopathologia · 2010Article
- The diagnosis of coccidioidomycosis.F1000 medicine reports · 2010Article
- Transmission of tropical and geographically restricted infections during solid-organ transplantation.Clinical microbiology reviews · 2008Review
- Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes.Current genomics · 2007Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A conventional nested PCR and a real-time LightCycler PCR assay for detection of Coccidioides posadasii DNA were designed and tested in 120 clinical strains. These had been isolated from 114 patients within 10 years in Monterrey, Nuevo Leon, Mexico, known to be endemic for coccidioidomycosis. The gene encoding the specific antigen 2/proline-rich antigen (Ag2/PRA) was used as a target. All strains were correctly identified, whereas DNA from related members of the family Onygenaceae remained negative. Melting curve analysis by LightCycler and sequencing of the 526-bp product of the first PCR demonstrated either 100% identity to the GenBank sequence of the Silveira strain, now known to be C. posadasii (accession number AF013256), or a single silent mutation at position 1228. Length determination of two microsatellite-containing loci (GAC and 621) identified all 120 isolates as C. posadasii. Specific DNA was amplified by conventional nested PCR from three microscopically spherule-positive paraffin-embedded tissue samples, whereas 20 human tissue samples positive for other dimorphic fungi remained negative. Additionally, the safety of each step of a modified commercially available DNA extraction procedure was evaluated by using 10 strains. At least three steps of the protocol were demonstrated to sufficiently kill arthroconidia. This safe procedure is applicable to cultures and to clinical specimens.
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