ArticleJournal of clinical microbiology2004
Identification of subgenus C adenoviruses by fiber-based multiplex PCR.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Molecular and epidemiological characterization of human adenovirus and classic human astrovirus in children with acute diarrhea in Shanghai, 2017-2018.BMC infectious diseases · 2021Article
- Adenovirus Isolated From a Cat Is Related to Human Adenovirus 1.Frontiers in microbiology · 2019Article
- Species differences in circulation and inflammatory responses in children with common respiratory adenovirus infections.Journal of medical virology · 2018Article
- A multigene typing system for human adenoviruses reveals a new genotype in a collection of Swedish clinical isolates.PloS one · 2018Article
- Quantitative PCR Detection and Characterisation of Human Adenovirus, Rotavirus and Hepatitis A Virus in Discharged Effluents of Two Wastewater Treatment Facilities in the Eastern Cape, South Africa.Food and environmental virology · 2016Article
- Quantitative real-time PCR assays for detection and type-specific identification of the endemic species C human adenoviruses.Journal of virological methods · 2016Article
- Identification of different respiratory viruses, after a cell culture step, by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS).Scientific reports · 2016Article
- Adenovirus: Epidemiology, Global Spread of Novel Serotypes, and Advances in Treatment and Prevention.Seminars in respiratory and critical care medicine · 2016Review
- Molecular identification of adenoviruses associated with respiratory infection in Egypt from 2003 to 2010.BMC infectious diseases · 2014Article
- Quantitative detection and characterization of human adenoviruses in the Buffalo River in the Eastern Cape Province of South Africa.Food and environmental virology · 2012Article
- Multiplexed Luminex xMAP assay for detection and identification of five adenovirus serotypes associated with epidemics of respiratory disease in adults.Journal of clinical microbiology · 2010Article
- [Application of molecular methods in the diagnosis and epidemiological study of viral respiratory infections].Enfermedades infecciosas y microbiologia clinica · 2008Review
- Detection of a broad range of human adenoviruses in respiratory tract samples using a sensitive multiplex real-time PCR assay.Journal of medical virology · 2008Article
- Co-infections of adenovirus species in previously vaccinated patients.Emerging infectious diseases · 2006Article
- PCR analysis of egyptian respiratory adenovirus isolates, including identification of species, serotypes, and coinfections.Journal of clinical microbiology · 2005Article
- Multiplex PCR assay for rapid identification of oculopathogenic adenoviruses by amplification of the fiber and hexon genes.Journal of clinical microbiology · 2005Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Subgenus C human adenoviruses, which include serotypes 1, 2, 5, and 6, are often associated with respiratory illness, ocular infections, gastroenteritis, and systemic infection among immunocompromised patients. To address the problems associated with the conventional typing methods, we developed a fiber-based multiplex PCR assay for simple and specific identification of adenovirus type 1, 2, 5, and 6 field isolates. To design type-specific primers, adenovirus type 1 and 6 fiber genes were sequenced. The assay correctly identified prototype strains of adenovirus serotypes 1, 2, 5, 6, as well as 21 previously typed adenovirus field isolates. Mixing two different prototype DNAs produced two amplicons of different lengths, thus clearly distinguishing the prototypes. The results correlated 100% with serological tests and 95% with the previously described PCR-restriction fragment length polymorphism method. The detection of dual infection is an added benefit of the assay. No nonspecific amplification was detected with other adenovirus serotypes or with nonadenoviral DNA. Our fiber-based multiplex PCR assay will provide a convenient tool for type-specific identification of subgenus C adenovirus isolates in various clinical situations and in epidemiological investigations and is a better alternative than the hexon-based assay.
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