Evidence map›Paper›PMID 14766828›Full record

ArticleJournal of clinical microbiology2004

Direct amplification and genotyping of Dientamoeba fragilis from human stool specimens.

Ron Peek, Freek R Reedeker, Tom van Gool

Abstract read
In one paragraph

Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

  1. Molecular epidemiology of Dientamoeba fragilis coinfections with Blastocystis sp. and Giardia duodenalis in symptomatic patients from Valencia, Spain.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026
    Article
  2. Molecular epidemiology and genotype diversity of Dientamoeba fragilis in a Czech hospital-based cohort: higher prevalence in paediatric patients and evidence of mixed G1/G2 infection in a single patient.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026
    Article
  3. Journal of medical microbiology · 2026
    Review
  4. Review
  5. Molecular detection ofFood and waterborne parasitology · 2025
    Article
  6. A cross-sectional survey ofOne health (Amsterdam, Netherlands) · 2024
    Article
  7. Article
  8. Article
  9. A Cross-Sectional Study on the Occurrence of the Intestinal Protist,International journal of molecular sciences · 2022
    Article
  10. Article
  11. Review
  12. Review
  13. Article
  14. Review
  15. Article
  16. Current treatment options for Dientamoeba fragilis infections.International journal for parasitology. Drugs and drug resistance · 2012
    Review
  17. Article
  18. In vitro susceptibility testing of Dientamoeba fragilis.Antimicrobial agents and chemotherapy · 2012
    Article
  19. Comparison of microscopy, two xenic culture techniques, conventional and real-time PCR for the detection of Dientamoeba fragilis in clinical stool samples.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010
    Article
  20. A review of the clinical presentation of dientamoebiasis.The American journal of tropical medicine and hygiene · 2010
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ron PeekDepartment of Medical Microbiology, Tropical Medicine and AIDS, University of Amsterdam, Amsterdam, The Netherlands. r.peek@amc.uva.nl
Freek R Reedeker
Tom van Gool

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dientamoeba fragilis is a globally occurring parasite that has been recognized as a causative agent of gastrointestinal symptoms. A single-round PCR was developed to detect D. fragilis DNA directly from human stool samples. The genetic diversity of D. fragilis from 93 patients and 6 asymptomatic carriers was examined by PCR followed by restriction fragment length polymorphism and sequencing of part of the small-subunit rRNA gene. The data show that D. fragilis sequences can be studied directly from fecal specimens despite the absence of a cyst stage and without the need for prior culturing. In addition, the results suggest strongly that D. fragilis shows remarkably little variation in its small-subunit rRNA gene.

Indexed as

AnimalsBase SequenceCloning, MolecularDientamoebaDientamoebiasisDNA, ProtozoanFecesGenetic VariationGenotypeHumansMolecular Sequence DataPolymerase Chain ReactionReference ValuesSequence AlignmentSequence Homology, Nucleic AcidDNA, Protozoan

Identifiers

PMID14766828
PMCPMC344490

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.