ArticleJournal of clinical microbiology2004
rpoB gene sequence-based identification of aerobic Gram-positive cocci of the genera Streptococcus, Enterococcus, Gemella, Abiotrophia, and Granulicatella.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Trial
- Invasive pneumococcal disease surveillance in Canada, 2023.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2026Article
- Discovery of IS1515 transposition into the Spn9802 fragment of Streptococcus pneumoniae and its implications for PCR-based diagnostic assays.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- High burden of viridans streptococcal endocarditis linked to multidrug resistance and regional risk factors: a molecular epidemiological report from Khyber Pakhtunkhwa, Pakistan.BMC microbiology · 2026Article
- A Septicemic Child due to a Novel Streptococcal Species,Case reports in infectious diseases · 2025Article
- Invasive pneumococcal disease surveillance in Canada, 2021-2022.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2024Article
- Investigating theMicrobiology (Reading, England) · 2022Article
- Invasive pneumococcal disease surveillance in Canada, 2020.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2022Article
- Whole-Genome Analysis of Streptococcus pneumoniae Serotype 4 Causing Outbreak of Invasive Pneumococcal Disease, Alberta, Canada.Emerging infectious diseases · 2021Article
- Optimization of the cultivation conditions ofBiotechnology reports (Amsterdam, Netherlands) · 2021Article
- Description and genomic characterization of Streptococcus symci sp. nov., isolated from a child's oropharynx.Antonie van Leeuwenhoek · 2021Article
- Vagococcus zengguangii sp. nov., isolated from yak faeces.Journal of microbiology (Seoul, Korea) · 2021Article
- Identification of Streptococcus pneumoniae and other Mitis streptococci: importance of molecular methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Review
- Identification of Clinically RelevantPathogens (Basel, Switzerland) · 2020Article
- Performance and Application of 16S rRNA Gene Cycle Sequencing for Routine Identification of Bacteria in the Clinical Microbiology Laboratory.Clinical microbiology reviews · 2020Review
- Partial rpoB Gene Sequencing Identification and Probiotic Potential of Floricoccus penangensis ML061-4 Isolated from Assam Tea (Camellia sinensis var. assamica).Scientific reports · 2019Article
- Alterations in the gut microbiome and metabolism with coronary artery disease severity.Microbiome · 2019Article
- Neonatal calf meningitis associated with Streptococcus gallolyticus subsp. gallolyticus.Folia microbiologica · 2019Article
- Development of a reference data set for assigningAntimicrobial resistance and infection control · 2019Article
- Streptococcus azizii sp. nov., isolated from naïve weanling mice.International journal of systematic and evolutionary microbiology · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a new molecular tool based on rpoB gene (encoding the beta subunit of RNA polymerase) sequencing to identify streptococci. We first sequenced the complete rpoB gene for Streptococcus anginosus, S. equinus, and Abiotrophia defectiva. Sequences were aligned with these of S. pyogenes, S. agalactiae, and S. pneumoniae available in GenBank. Using an in-house analysis program (SVARAP), we identified a 740-bp variable region surrounded by conserved, 20-bp zones and, by using these conserved zones as PCR primer targets, we amplified and sequenced this variable region in an additional 30 Streptococcus, Enterococcus, Gemella, Granulicatella, and Abiotrophia species. This region exhibited 71.2 to 99.3% interspecies homology. We therefore applied our identification system by PCR amplification and sequencing to a collection of 102 streptococci and 60 bacterial isolates belonging to other genera. Amplicons were obtained in streptococci and Bacillus cereus, and sequencing allowed us to make a correct identification of streptococci. Molecular signatures were determined for the discrimination of closely related species within the S. pneumoniae-S. oralis-S. mitis group and the S. agalactiae-S. difficile group. These signatures allowed us to design a S. pneumoniae-specific PCR and sequencing primer pair.
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