ArticleApplied and environmental microbiology2004
Multi-virulence-locus sequence typing of Listeria monocytogenes.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed.
- Bacterial Antimicrobial Resistance in Meat Products-Current Concepts.Foods (Basel, Switzerland) · 2025Review
- Genotypic Analysis of Candida tropicalis Clinical Isolates From Korea via Multilocus Sequence Typing.MicrobiologyOpen · 2025Article
- Genetic Diversity, Virulence Factors and Antibiotic Resistance ofPathogens (Basel, Switzerland) · 2024Article
- Prevalence and Antimicrobial Resistance ofFoods (Basel, Switzerland) · 2024Article
- Characterization ofAntibiotics (Basel, Switzerland) · 2024Article
- Impaired meningeal lymphatic drainage inFrontiers in immunology · 2024Article
- Current methodologies available to evaluate the virulence potential amongFrontiers in microbiology · 2024Review
- Pseudo-outbreak ofInfection control and hospital epidemiology · 2023Article
- The Prevalence and Antibiotic-Resistant ofFoods (Basel, Switzerland) · 2023Review
- Review
- Frozen Vegetable Processing Plants Can Harbour DiverseFoods (Basel, Switzerland) · 2022Article
- Diversity ofFoods (Basel, Switzerland) · 2021Article
- A review of Listeria monocytogenes from meat and meat products: Epidemiology, virulence factors, antimicrobial resistance and diagnosis.The Onderstepoort journal of veterinary research · 2020Review
- Prevalence, Genotypic Characteristics and Antibiotic Resistance ofFrontiers in microbiology · 2019Article
- Importance ofIranian journal of veterinary research · 2019Review
- Laboratory Methods in Molecular Epidemiology: Bacterial Infections.Microbiology spectrum · 2018Review
- Phylogenetics of Mycoplasma hominis clinical strains associated with gynecological infections or infertility as disclosed by an expanded multilocus sequence typing scheme.Scientific reports · 2018Article
- Use of omics methods for the advancement of food quality and food safety.Animal frontiers : the review magazine of animal agriculture · 2018Article
- Epidemiological burden ofIranian journal of basic medical sciences · 2018Review
- Genetic Analysis of the Listeria Pathogenicity Island 1 of Listeria monocytogenes 1/2a and 4b Isolates.Current microbiology · 2018Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multi-virulence-locus sequence typing (MVLST) scheme was developed for subtyping Listeria monocytogenes, and the results obtained using this scheme were compared to those of pulsed-field gel electrophoresis (PFGE) and the published results of other typing methods, including ribotyping (RT) and multilocus sequence typing (MLST). A set of 28 strains (eight different serotypes and three known genetic lineages) of L. monocytogenes was selected from a strain collection (n > 1,000 strains) to represent the genetic diversity of this species. Internal fragments (ca. 418 to 469 bp) of three virulence genes (prfA, inlB, and inlC) and three virulence-associated genes (dal, lisR, and clpP) were sequenced and analyzed. Multiple DNA sequence alignment identified 10 (prfA), 19 (inlB), 13 (dal), 10 (lisR), 17 (inlC), and 16 (clpP) allelic types and a total of 28 unique sequence types. Comparison of MVLST with automated EcoRI-RT and PFGE with ApaI enzymatic digestion showed that MVLST was able to differentiate strains that were indistinguishable by RT (13 ribotypes; discrimination index = 0.921) or PFGE (22 profiles; discrimination index = 0.970). Comparison of MVLST with housekeeping-gene-based MLST analysis showed that MVLST provided higher discriminatory power for serotype 1/2a and 4b strains than MLST. Cluster analysis based on the intragenic sequences of the selected virulence genes indicated a strain phylogeny closely related to serotypes and genetic lineages. In conclusion, MVLST may improve the discriminatory power of MLST and provide a convenient tool for studying the local epidemiology of L. monocytogenes.
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