ArticleThe Biochemical journal2004
Location of promoter elements necessary and sufficient to direct testis-specific expression of the Hst70/Hsp70.2 gene.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 20 citations in OpenAlex.
- Evolution of a New Testis-Specific Functional Promoter Within the Highly ConservedFrontiers in genetics · 2021Article
- CircNAPEPLD is expressed in human and murine spermatozoa and physically interacts with oocyte miRNAs.RNA biology · 2019Article
- Expression, function, and regulation of the testis-enriched heat shock HSPA2 gene in rodents and humans.Cell stress & chaperones · 2015Review
- A-MYB (MYBL1) stimulates murine testis-specific Ldhc expression via the cAMP-responsive element (CRE) site.Biology of reproduction · 2012Article
- Differential expression of HSPA1 and HSPA2 proteins in human tissues; tissue microarray-based immunohistochemical study.Histochemistry and cell biology · 2011Article
- Regulation of murine lactate dehydrogenase C (Ldhc) gene expression.Biology of reproduction · 2008Article
- Transcripts associated with Prdx6 (peroxiredoxin 6) and related genes in mouse.Mammalian genome : official journal of the International Mammalian Genome Society · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rat Hst70 gene and its mouse counterpart Hsp70.2 are expressed specifically in pachytene primary spermatocytes and spermatids. Here we demonstrate that a 165 bp fragment of the Hst70 gene promoter, containing the T1 transcription start site region, entire exon 1 and 42 bp 5' region of the intron, is sufficient to drive testis-specific expression of the chloramphenicol acetyltransferase reporter gene in transgenic mice with the same developmentally regulated pattern as the endogenous Hsp70.2 gene. We show further that high-level tissue-specific gene expression requires additional sequences localized upstream of the T2 transcription start site. Electrophoretic mobility-shift assay analysis revealed that only testes of juvenile rats, when Hst70 gene expression is repressed, contain proteins that specifically bind to the Oct (octamer) sequence localized directly downstream of the T1 site.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.