ArticleMolecular biotechnology2004
Rat liver-targeted naked plasmid DNA transfer by tail vein injection.
Article in Molecular biotechnology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
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- Insulin-like growth factor binding protein-related protein 1 (IGFBPrP1) contributes to liver inflammation and fibrosis via activation of the ERK1/2 pathway.Hepatology international · 2015Article
- Mest attenuates CCl4-induced liver fibrosis in rats by inhibiting the Wnt/β-catenin signaling pathway.Gut and liver · 2014Article
- A method to facilitate and monitor expression of exogenous genes in the rat kidney using plasmid and viral vectors.American journal of physiology. Renal physiology · 2013Article
- Gene transfer of c-met confers protection against D-galactosamine/lipopolysaccharide-induced acute liver failure.Digestive diseases and sciences · 2012Article
- Viral and nonviral delivery systems for gene delivery.Advanced biomedical research · 2012Article
- Nonviral gene delivery: what we know and what is next.The AAPS journal · 2007Review
- Rat kidney-targeted naked plasmid DNA transfer by retrograde injection into the renal vein.Molecular biotechnology · 2004Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High levels of foreign gene expression in mouse hepatocytes can be achieved by "hydrodynamics-based transfection," the rapid injection of a large volume of a naked deoxyribonucleic acid (DNA) solution into the tail vein. Rats are more tolerant of the frequent phlebotomies required for monitoring blood parameters than mice and, thus, are more suitable for some biomedical research. Recently, we demonstrated that hydrodynamics-based transfection can also be used to deliver naked plasmid DNA into the normal rat, which is more than 10 times larger than the mouse. We performed the tail vein injection using a syringe with a winged needle equipped with an external tube. Injection of a lac Z expression plasmid, pCAGGS-lac Z by this technique resulted in the exclusive detection of beta-galactosidase in the liver. We also injected a rat erythropoietin (Epo) expression plasmid, pCAGGS-Epo (800 microg). Maximal Epo gene expression was achieved when a 25-mL injection volume (approx 100 mL/kg body wt) was transferred within 15 s.
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