ReviewMolecular biotechnology2004
Pre-PCR processing: strategies to generate PCR-compatible samples.
Review in Molecular biotechnology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 135 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
135 citing papers in PubMed, 1 synthesis or guideline pooled it.
- PCR using blood for diagnosis of invasive pneumococcal disease: systematic review and meta-analysis.Journal of clinical microbiology · 2010Pooled it
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- Evaluating the mechanism of indigo-mediated PCR inhibition and validation of nano-based mitigation for forensic STR profiling.International journal of legal medicine · 2026Article
- Monitoring of Adenoviruses in Water and Wastewater through Applications of PCR Based Methods.Indian journal of microbiology · 2025Review
- Validation of an Optimised Method for Quantitative Detection of Hepatitis E Virus in Pork Sausage.Food and environmental virology · 2025Article
- Recombinase polymerase amplification assay for sensitive and rapid detection of invasive fall armyworm, Spodoptera frugiperda.Scientific reports · 2025Article
- Development of point-of-need colourimetric, isothermal diagnostic assays for specific detection of Bacillus subtilis using shikimate dehydrogenase gene.Folia microbiologica · 2025Article
- Elucidation of peptide screen for targeted identification of Yersinia pestis by nano-liquid chromatography tandem mass spectrometry.Scientific reports · 2025Article
- Machine learning-driven dissection of the obesity-ccRCC interface: FCGR2A emerges as a central coordinator of tumor-immune crosstalk.Frontiers in oncology · 2025Article
- A simple and cost-effective real-time PCR method using diluted and heat-treated whole blood lysate.Scientific reports · 2024Article
- Enhanced Recovery and Detection of Highly Infectious Animal Disease Viruses by Virus Capture Using NanotrapViruses · 2024Article
- Development of recombinase polymerase amplification-based colorimetric detection assay for rapid identification of invasive cassava mealybug,Saudi journal of biological sciences · 2024Article
- Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond.Clinical microbiology reviews · 2024Review
- Tackling Infectious Diseases with Rapid Molecular Diagnosis and Innovative Prevention.Infectious disease reports · 2024Review
- Optimization of the STARlet workflow for semi-automatic SARS-CoV-2 screening of swabs and deep respiratory materials using the RealAccurate Quadruplex SARS-CoV-2 PCR kit and Allplex SARS-CoV-2 PCR kit.Microbiology spectrum · 2024Article
- Rapid Minimum Inhibitory Concentration (MIC) Analysis Using Lyophilized Reagent Beads in a Novel Multiphase, Single-Vessel Assay.Antibiotics (Basel, Switzerland) · 2023Article
- Diagnostic Evaluation of the IS1081-Targeted Real-Time PCR for Detection ofPathogens (Basel, Switzerland) · 2023Article
- Rapid RT-PCR identification of SARS-CoV-2 in screening donors of fecal microbiota transplantation.Heliyon · 2023Article
- Comparison of diaphragm meat juice and muscle swab samples to spleen and spleen swab samples for the detection of African swine fever viral nucleic acid.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023Article
- Cost-Effective Next Generation Sequencing-Based STR Typing with Improved Analysis of Minor, Degraded and Inhibitor-Containing DNA Samples.International journal of molecular sciences · 2023Article
75 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polymerase chain reaction (PCR) is recognized as a rapid, sensitive, and specific molecular diagnostic tool for the analysis of nucleic acids. However, the sensitivity and kinetics of diagnostic PCR may be dramatically reduced when applied directly to biological samples, such as blood and feces, owing to PCR-inhibitory components. As a result, pre-PCR processing procedures have been developed to remove or reduce the effects of PCR inhibitors. Pre-PCR processing comprises all steps prior to the detection of PCR products, that is, sampling, sample preparation, and deoxyribonucleic acid (DNA) amplification. The aim of pre-PCR processing is to convert a complex biological sample with its target nucleic acids/cells into PCR-amplifiable samples by combining sample preparation and amplification conditions. Several different pre-PCR processing strategies are used: (1) optimization of the DNA amplification conditions by the use of alternative DNA polymerases and/or amplification facilitators, (2) optimization of the sample preparation method, (3) optimization of the sampling method, and (4) combinations of the different strategies. This review describes different pre-PCR processing strategies to circumvent PCR inhibition to allow accurate and precise DNA amplification.
Indexed as
Identifiers
14764939What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.