ArticlePlant physiology2004
Cloning, characterization, and immunolocalization of a mycorrhiza-inducible 1-deoxy-d-xylulose 5-phosphate reductoisomerase in arbuscule-containing cells of maize.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 112 citations in OpenAlex.
- Cloning and functional analysis of the DXR gene and promoter region in Osmanthus fragrans var. semperflorens.Functional & integrative genomics · 2023Article
- Site-directed mutagenesis identified the key active site residues of 2,3-oxidosqualene cyclase HcOSC6 responsible for cucurbitacins biosynthesis inFrontiers in plant science · 2023Article
- Plant environmental sensing relies on specialized plastids.Journal of experimental botany · 2022Review
- Volatile organic compounds shape belowground plant-fungi interactions.Frontiers in plant science · 2022Review
- Visualising an invisible symbiosis.Plants, people, planet · 2021Article
- Plant apocarotenoids: from retrograde signaling to interspecific communication.The Plant journal : for cell and molecular biology · 2021Review
- AM-Induced Alteration in the Expression of Genes, Encoding Phosphorus Transporters and Enzymes of Carbohydrate Metabolism inPlants (Basel, Switzerland) · 2020Article
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- Strategies for the production of biochemicals in bioenergy crops.Biotechnology for biofuels · 2020Review
- SEED CAROTENOID DEFICIENT Functions in Isoprenoid Biosynthesis via the Plastid MEP Pathway.Plant physiology · 2019Article
- Solanesol Biosynthesis in Plants.Molecules (Basel, Switzerland) · 2017Review
- Terpenoids in plant and arbuscular mycorrhiza-reinforced defence against herbivorous insects.Annals of botany · 2017Review
- Molecular Cloning and Characterization of DXS and DXR Genes in the Terpenoid Biosynthetic Pathway of Tripterygium wilfordii.International journal of molecular sciences · 2015Article
- Article
- The myth of interconnected plastids and related phenomena.Protoplasma · 2015Article
- Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal.Frontiers in plant science · 2015Review
- DELLA proteins regulate arbuscule formation in arbuscular mycorrhizal symbiosis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Arabidopsis J-protein J20 delivers the first enzyme of the plastidial isoprenoid pathway to protein quality control.The Plant cell · 2013Article
- Expression pattern of fifteen genes of non-mevalonate (MEP) and mevalonate (MVA) pathways in different tissues of endangered medicinal herb Picrorhiza kurroa with respect to picrosides content.Molecular biology reports · 2013Article
- Characterization and functional analysis of the genes encoding 1-deoxy-D-xylulose-5-phosphate reductoisomerase and 1-deoxy-D-xylulose-5-phosphate synthase, the two enzymes in the MEP pathway, from Amomum villosum Lour.Molecular biology reports · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Colonization of plant roots by symbiotic arbuscular mycorrhizal fungi frequently leads to the accumulation of several apocarotenoids. The corresponding carotenoid precursors originate from the plastidial 2-C-methyl-d-erythritol 4-phosphate pathway. We have cloned and characterized 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXR), catalyzing the first committed step of the pathway, from maize (Zea mays). Functional identification was accomplished by heterologous expression of sequences coding for the mature protein in Escherichia coli. DXR is up-regulated in maize roots during mycorrhization as shown at transcript and protein levels, but is also abundant in leaves and young seedlings. Inspection of sequenced genomes and expressed sequence tag (EST) databases argue for a single-copy DXR gene. Immunolocalization studies in mycorrhizal roots using affinity-purified antibodies revealed a DXR localization in plastids around the main symbiotic structures, the arbuscules. DXR protein accumulation is tightly correlated with arbuscule development. The highest level of DXR protein is reached around maturity and initial senescence of these structures. We further demonstrate the formation of a DXR-containing plastidial network around arbuscules, which is highly interconnected in the mature, functional state of the arbuscules. Our findings imply a functional role of a still unknown nature for the apocarotenoids or their respective carotenoid precursors in the arbuscular life cycle.
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