ArticlePlant physiology2004
Two distinct signaling pathways participate in auxin-induced swelling of pea epidermal protoplasts.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 86 citations in OpenAlex.
- Aggregates-on-chip: a microfluidic device for crowding plant cells in culture.Frontiers in plant science · 2026Article
- Plants maximise chloride uptake during early vegetative development to stimulate cell expansion, maturation of the photosynthetic apparatus, and growth.The Plant journal : for cell and molecular biology · 2025Article
- Have All of the Phytohormonal Properties of Melatonin Been Verified?International journal of molecular sciences · 2024Review
- No Home without Hormones: How Plant Hormones Control Legume Nodule Organogenesis.Plant communications · 2020Review
- Chloride as a Beneficial Macronutrient in Higher Plants: New Roles and Regulation.International journal of molecular sciences · 2019Review
- Auxin Signaling.Plant physiology · 2018Review
- Protoplast Swelling and Hypocotyl Growth Depend on Different Auxin Signaling Pathways.Plant physiology · 2017Article
- Leaf expansion inPhysiologia plantarum · 2017Article
- Auxin flow-mediated competition between axillary buds to restore apical dominance.Scientific reports · 2016Article
- Article
- Chloride regulates leaf cell size and water relations in tobacco plants.Journal of experimental botany · 2016Article
- Pavement cells: a model system for non-transcriptional auxin signalling and crosstalks.Journal of experimental botany · 2015Review
- Embryo-lethal phenotypes in early abp1 mutants are due to disruption of the neighboring BSM gene.F1000Research · 2015Article
- Novel ABP1-TMK auxin sensing system controls ROP GTPase-mediated interdigitated cell expansion in Arabidopsis.Small GTPases · 2014Review
- Ca(2+)-Transport through Plasma Membrane as a Test of Auxin Sensitivity.Plants (Basel, Switzerland) · 2014Article
- Calcium: The Missing Link in Auxin Action.Plants (Basel, Switzerland) · 2013Review
- Auxin activates the plasma membrane H+-ATPase by phosphorylation during hypocotyl elongation in Arabidopsis.Plant physiology · 2012Article
- Short-term aluminium-induced changes in barley root tips.Protoplasma · 2011Article
- Is ABP1 an auxin receptor yet?Molecular plant · 2011Review
- Uniform auxin triggers the Rho GTPase-dependent formation of interdigitation patterns in pavement cells.Small GTPases · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protoplast swelling was used to investigate auxin signaling in the growth-limiting stem epidermis. The protoplasts of epidermal cells were isolated from elongating internodes of pea (Pisum sativum). These protoplasts swelled in response to auxin, providing the clearest evidence that the epidermis can directly perceive auxin. The swelling response to the natural auxin IAA showed a biphasic dose response curve but that to the synthetic auxin 1-naphthalene acetic acid (NAA) showed a simple bell-shaped dose response curve. The responses to IAA and NAA were further analyzed using antibodies raised against ABP1 (auxin-binding protein 1), and their dependency on extracellular ions was investigated. Two signaling pathways were resolved for IAA, an ABP1-dependent pathway and an ABP1-independent pathway that is much more sensitive to IAA than the former. The response by the ABP1 pathway was eliminated by anti-ABP1 antibodies, had a higher sensitivity to NAA, and did not depend on extracellular Ca(2+). In contrast, the response by the non-ABP1 pathway was not affected by anti-ABP1 antibodies, had no sensitivity to NAA, and depended on extracellular Ca(2+). The swelling by either pathway required extracellular K(+) and Cl(-). The auxin-induced growth of pea internode segments showed similar response patterns, including the occurrence of two peaks in the dose response curve for IAA and the difference in Ca(2+) requirements. It is suggested that two signaling pathways participate in auxin-induced internode growth and that the non-ABP1 pathway is more likely to be involved in the control of growth by constitutive concentrations of endogenous auxin.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.