ArticleEuropean biophysics journal : EBJ2004
Resolving the molecular structure of microtubules under physiological conditions with scanning force microscopy.
Article in European biophysics journal : EBJ, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- A molecular trap inside microtubules probes luminal access by soluble proteins.Nature chemical biology · 2021Article
- Lattice defects induce microtubule self-renewal.Nature physics · 2019Article
- Article
- Article
- Non-equilibrium assembly of microtubules: from molecules to autonomous chemical robots.Chemical Society reviews · 2017Review
- Tubulin acetylation protects long-lived microtubules against mechanical ageing.Nature cell biology · 2017Article
- Single Molecule Investigation of Kinesin-1 Motility Using Engineered Microtubule Defects.Scientific reports · 2017Article
- Microtubule Defects Influence Kinesin-Based Transport In Vitro.Biophysical journal · 2016Article
- Calcium Promotes the Formation of Syntaxin 1 Mesoscale Domains through Phosphatidylinositol 4,5-Bisphosphate.The Journal of biological chemistry · 2016Article
- Microtubules self-repair in response to mechanical stress.Nature materials · 2015Article
- Swelling and softening of the cowpea chlorotic mottle virus in response to pH shifts.Biophysical journal · 2015Article
- Tau protein diffuses along the microtubule lattice.The Journal of biological chemistry · 2012Article
- Kinesin walks the line: single motors observed by atomic force microscopy.Biophysical journal · 2011Article
- Moving into the cell: single-molecule studies of molecular motors in complex environments.Nature reviews. Molecular cell biology · 2011Review
- Malaria parasite actin polymerization and filament structure.The Journal of biological chemistry · 2010Article
- Mechanics of microtubules: effects of protofilament orientation.Biophysical journal · 2010Article
- Probing nanomechanical properties from biomolecules to living cells.Pflugers Archiv : European journal of physiology · 2008Review
- Analysis of nematode mechanics by piezoresistive displacement clamp.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Elastic response, buckling, and instability of microtubules under radial indentation.Biophysical journal · 2006Article
- Thermal fluctuations of grafted microtubules provide evidence of a length-dependent persistence length.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have imaged microtubules, essential structural elements of the cytoskeleton in eukaryotic cells, in physiological conditions by scanning force microscopy. We have achieved molecular resolution without the use of cross-linking and chemical fixation methods. With tip forces below 0.3 nN, protofilaments with approximately 6 nm separation could be clearly distinguished. Lattice defects in the microtubule wall were directly visible, including point defects and protofilament separations. Higher tip forces destroyed the top half of the microtubules, revealing the inner surface of the substrate-attached protofilaments. Monomers could be resolved on these inner surfaces.
Indexed as
Identifiers
14762705What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.