ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2004
Intracellular association of glycine receptor with gephyrin increases its plasma membrane accumulation rate.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 79 citations in OpenAlex.
- Synaptic vs. Non-Synaptic Glycine Receptors: Physiological Role and Implications in Alzheimer's Disease Pathology.International journal of molecular sciences · 2026Review
- GDNF Increases Inhibitory Synaptic Drive on Principal Neurons in the Hippocampus via Activation of the Ret Pathway.International journal of molecular sciences · 2022Article
- Binding of gephyrin to microtubules is regulated by its phosphorylation at Ser270.Histochemistry and cell biology · 2021Article
- Reciprocal stabilization of glycine receptors and gephyrin scaffold proteins at inhibitory synapses.Biophysical journal · 2021Article
- Gephyrin-mediated formation of inhibitory postsynaptic density sheet via phase separation.Cell research · 2021Article
- New approaches for solving old problems in neuronal protein trafficking.Molecular and cellular neurosciences · 2018Review
- Assembly and maintenance of GABAergic and Glycinergic circuits in the mammalian nervous system.Neural development · 2018Review
- Heterogeneous Signaling at GABA and Glycine Co-releasing Terminals.Frontiers in synaptic neuroscience · 2018Article
- Impaired Glycine Receptor Trafficking in Neurological Diseases.Frontiers in molecular neuroscience · 2018Review
- Glycine Receptor Activation Impairs ATP-Induced Calcium Transients in Cultured Cortical Astrocytes.Frontiers in molecular neuroscience · 2017Article
- Defects of the Glycinergic Synapse in Zebrafish.Frontiers in molecular neuroscience · 2016Review
- Differential GABAergic and glycinergic inputs of inhibitory interneurons and Purkinje cells to principal cells of the cerebellar nuclei.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Synaptic control of secretory trafficking in dendrites.Cell reports · 2014Article
- Gephyrin: a master regulator of neuronal function?Nature reviews. Neuroscience · 2014Review
- Article
- Argonaute2 regulates the pancreatic β-cell secretome.Molecular & cellular proteomics : MCP · 2013Article
- Molecular and functional heterogeneity of GABAergic synapses.Cellular and molecular life sciences : CMLS · 2012Review
- Gephyrin, the enigmatic organizer at GABAergic synapses.Frontiers in cellular neuroscience · 2012Article
- Heat shock cognate protein 70 regulates gephyrin clustering.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Alterations of GABAergic signaling in autism spectrum disorders.Neural plasticity · 2011Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gephyrin, a tubulin-binding protein, is the core of inhibitory postsynaptic scaffolds stabilizing glycine receptors (GlyRs) and/or GABA(A) receptors. Previous ultrastructural studies in vivo and in vitro have reported a localization of gephyrin to intracellular cisternas during development or after glycinergic denervation (Seitanidou et al., 1992; Colin et al., 1996, 1998). These data were compatible with a traffic of this cytoplasmic, but membrane-associated, protein together with membrane proteins such as GlyR after exocytosis and/or endocytosis pathways. We have now investigated the consequences of a GlyR-gephyrin interaction on the localization and the dynamics of these two molecules in African green monkey kidney cells (COS-7) cells and in neurons transfected with green fluorescent protein-tagged-gephyrin and myc-tagged GlyR alpha1 subunits. In these experiments, myc-tagged GlyR alpha1 contained, or did not contain, the gephyrin-binding sequence (betagb) of the GlyR beta subunit. We report here that GlyR-gephyrin interaction localizes gephyrin to GlyR-containing organelles. Videomicroscopy and nocodazole treatment indicate that the movements of these vesicles are microtubule dependent. Expressing GlyR alpha1 with a thrombin cleavage site between the myc-tag and the N terminal of the GlyR alpha1 subunit (Rosenberg et al., 2001) allowed monitoring of newly inserted receptors in the cell surface. Using temperature changes to block GlyR in, and then release it from, the trans-Golgi network, we show that gephyrin accelerates the accumulation of GlyR at the cell surface. Therefore, our data strongly suggest that some GlyR clusters are associated with gephyrin on their way to the cell surface and that this association increases the accumulation of GlyR at the plasma membrane.
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