ArticleInternational journal of legal medicine2004
A multiplex allele-specific primer extension assay for forensically informative SNPs distributed throughout the mitochondrial genome.
Article in International journal of legal medicine, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 144 citations in OpenAlex.
- Improving phylogenetic resolution of the Lamiales using the complete plastome sequences of six Penstemon species.PloS one · 2021Article
- Forensically relevant SNaPshotInternational journal of legal medicine · 2017Review
- Studying Bordetella pertussis populations by use of SNPeX, a simple high-throughput single nucleotide polymorphism typing method.Journal of clinical microbiology · 2015Article
- AmericaPlex26: a SNaPshot multiplex system for genotyping the main human mitochondrial founder lineages of the Americas.PloS one · 2014Article
- A high-throughput Sanger strategy for human mitochondrial genome sequencing.BMC genomics · 2013Article
- Preamplification procedure for the analysis of ancient DNA samples.TheScientificWorldJournal · 2013Article
- SNaPshot Assay in Quantitative Detection of Allelic Nondisjunction in Down Syndrome.Genetic testing and molecular biomarkers · 2012Article
- Multiplex genotyping system for efficient inference of matrilineal genetic ancestry with continental resolution.Investigative genetics · 2011Article
- The GHEP-EMPOP collaboration on mtDNA population data--A new resource for forensic casework.Forensic science international. Genetics · 2011Article
- Molecular database for classifying Shorea species (Dipterocarpaceae) and techniques for checking the legitimacy of timber and wood products.Journal of plant research · 2011Article
- Multiplex analysis of mitochondrial DNA pathogenic and polymorphic sequence variants.Biological chemistry · 2010Article
- Single PCR multiplex SNaPshot reaction for detection of eleven blood group nucleotide polymorphisms: optimization, validation, and one year of routine clinical use.The Journal of molecular diagnostics : JMD · 2010Article
- Typing of 24 mtDNA SNPs in a Chinese population using SNaPshot minisequencing.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2010Article
- Subtyping of Y-chromosomal haplogroup E-M78 (E1b1b1a) by SNP assay and its forensic application.International journal of legal medicine · 2009Article
- Evaluation of single-nucleotide primer extension for detection and typing of phylogenetic markers used for investigation of microbial communities.Applied and environmental microbiology · 2009Article
- A rapid mtDNA assay of 22 SNPs in one multiplex reaction increases the power of forensic testing in European Caucasians.International journal of legal medicine · 2008Article
- Resolving individuals contributing trace amounts of DNA to highly complex mixtures using high-density SNP genotyping microarrays.PLoS genetics · 2008Article
- Multiplex mutagenically separated polymerase chain reaction assay for rapid detection of human mitochondrial DNA variations in coding area.Croatian medical journal · 2008Article
- Single-nucleotide primer extension assay for detection and sequence typing of "Dehalococcoides" spp.Applied and environmental microbiology · 2008Article
- STRs vs. SNPs: thoughts on the future of forensic DNA testing.Forensic science, medicine, and pathology · 2007Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The typing of single nucleotide polymorphisms (SNPs) located throughout the mitochondrial genome (mtGenome) can help resolve individuals with an identical HV1/HV2 mitotype. A set of 11 SNPs selected for distinguishing individuals of the most common Caucasian HV1/HV2 mitotype were incorporated in an allele specific primer extension assay. The assay was optimized for multiplex detection of SNPs at positions 3010, 4793, 10211, 5004, 7028, 7202, 16519, 12858, 4580, 477 and 14470 in the mtGenome. Primers were designed to allow for simultaneous PCR amplification of 11 unique regions in the mtGenome and subsequent primer extension. By enzymatically incorporating fluorescently labeled dideoxynucleotides (ddNTPs) onto the 3' end of the extension primer, detection can be accomplished with a capillary-based electrophoresis (CE) platform common in most forensic laboratories. The electrophoretic mobility for the extension primers was compared in denaturing POP4 and POP6 CE running buffers. Empirical adjustment of extension primer concentrations resulted in even signal intensity for the 11 loci probed. We demonstrate that the assay performs well for heteroplasmy and mixture detection, and for typical mtDNA casework samples with highly degraded DNA.
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