ArticleNucleic acids research2004
The Trypanosoma brucei spliced leader RNA and rRNA gene promoters have interchangeable TbSNAP50-binding elements.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- TBP and SNAP50 transcription factors bind specifically to the Pr77 promoter sequence from trypanosomatid non-LTR retrotransposons.Parasites & vectors · 2021Article
- Transcription Factor Analysis in Trypanosomatids.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Transcriptional regulation of snRNAs and its significance for plant development.Journal of plant research · 2017Review
- Dynein Light Chain LC8 Is Required for RNA Polymerase I-Mediated Transcription in Trypanosoma brucei, Facilitating Assembly and Promoter Binding of Class I Transcription Factor A.Molecular and cellular biology · 2016Article
- Ribosomal RNA gene transcription in trypanosomes.Parasitology research · 2014Review
- TbENF is an essential TbTFIIB-interacting trypanosomatid-specific factor.Molecular and biochemical parasitology · 2012Article
- Development of an efficient in vitro transcription system for bloodstream form Trypanosoma brucei reveals life cycle-independent functionality of class I transcription factor A.Molecular and biochemical parasitology · 2012Article
- Transcription by the multifunctional RNA polymerase I in Trypanosoma brucei functions independently of RPB7.Molecular and biochemical parasitology · 2011Article
- Essential role of a trypanosome U4-specific Sm core protein in small nuclear ribonucleoprotein assembly and splicing.Eukaryotic cell · 2010Article
- Structure of the C-terminal domain of transcription factor IIB from Trypanosoma brucei.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Special Sm core complex functions in assembly of the U2 small nuclear ribonucleoprotein of Trypanosoma brucei.Eukaryotic cell · 2009Article
- Histone acetylations mark origins of polycistronic transcription in Leishmania major.BMC genomics · 2009Article
- Histone acetylation and methylation at sites initiating divergent polycistronic transcription in Trypanosoma cruzi.The Journal of biological chemistry · 2008Article
- Multifunctional class I transcription in Trypanosoma brucei depends on a novel protein complex.The EMBO journal · 2007Article
- Active RNA polymerase I of Trypanosoma brucei harbors a novel subunit essential for transcription.Molecular and cellular biology · 2007Article
- Spliced leader RNA gene transcription in Trypanosoma brucei requires transcription factor TFIIH.Eukaryotic cell · 2007Article
- Insect small nuclear RNA gene promoters evolve rapidly yet retain conserved features involved in determining promoter activity and RNA polymerase specificity.Nucleic acids research · 2007Article
- Sm core variation in spliceosomal small nuclear ribonucleoproteins from Trypanosoma brucei.The EMBO journal · 2006Article
- Repression of polymerase I-mediated gene expression at Trypanosoma brucei telomeres.EMBO reports · 2006Article
- A TFIIB-like protein is indispensable for spliced leader RNA gene transcription in Trypanosoma brucei.Nucleic acids research · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the protist parasite Trypanosoma brucei, the small nuclear spliced leader (SL) RNA and the large rRNAs are key molecules for mRNA maturation and protein synthesis, respectively. The SL RNA gene (SLRNA) promoter recruits RNA polymerase II and consists of a bipartite upstream sequence element (USE) and an element close to the transcription initiation site. Here, we analyzed the distal part of the ribosomal (RRNA) promoter and identified two sequence blocks which, in reverse orientation, closely resemble the SLRNA USE by both sequence and spacing. A detailed mutational analysis revealed that the ribosomal (r)USE is essential for efficient RRNA transcription in vivo and that it functions in an orientation-dependent manner. Moreover, we showed that USE and rUSE are functionally interchangeable and that rUSE stably interacted with an essential factor of SLRNA transcription. Finally, we demonstrated that the T.brucei homolog of the recently characterized transcription factor p57 of the related organism Leptomonas seymouri specifically bound to USE and rUSE. Since p57 and its T.brucei counterpart are homologous to SNAP50, a component of the human small nuclear RNA gene activation protein complex (SNAPc), both SLRNA and RRNA transcription in T.brucei may depend on a SNAPc-like transcription factor.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.