ArticlePlant molecular biology2003
Characterization of two phosphate transporters from barley; evidence for diverse function and kinetic properties among members of the Pht1 family.
Article in Plant molecular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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60 citing papers in PubMed, 179 citations in OpenAlex.
- Identification of the phosphate transporter 1 family genes in the Eucalyptus grandis genome and their expression under different phosphate regimes.BMC genomics · 2026Article
- Beyond phosphate uptake: PT4/PT11 as key functional components linking phosphate acquisition, nutrient exchange, and arbuscule maintenance in arbuscular mycorrhizal symbiosis.Frontiers in plant science · 2026Review
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- Characterizing the wheat (Frontiers in plant science · 2025Article
- Identification of GmPT proteins and investigation of their expressions in response to abiotic stress in soybean.Planta · 2024Article
- Isolation and Characterization ofPlants (Basel, Switzerland) · 2023Article
- Five Post-Translational Modification Residues of CmPT2 Play Key Roles in Yeast and Rice.International journal of molecular sciences · 2023Article
- Adaptive strategies of plants to conserve internal phosphorus under P deficient condition to improve P utilization efficiency.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Review
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- Fine-tuning the transcriptional regulatory model of adaptation response to phosphate stress in maize (Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- The rice phosphate transporter OsPHT1;7 plays a dual role in phosphorus redistribution and anther development.Plant physiology · 2022Article
- Mechanisms for improving phosphorus utilization efficiency in plants.Annals of botany · 2022Review
- Understanding the Adaptive Mechanisms of Plants to Enhance Phosphorus Use Efficiency on Podzolic Soils in Boreal Agroecosystems.Frontiers in plant science · 2022Review
- Article
- Genome-Wide Analysis of the Five Phosphate Transporter Families inInternational journal of molecular sciences · 2020Article
- Genome-Wide Analysis of Phosphorus Transporter Genes inInternational journal of molecular sciences · 2020Article
- Contribution of External and Internal Phosphorus Sources to Grain P Loading in Durum Wheat (Frontiers in plant science · 2020Article
- Adaptation of Foxtail Millet (Frontiers in plant science · 2020Review
- OsPHT1;3 Mediates Uptake, Translocation, and Remobilization of Phosphate under Extremely Low Phosphate Regimes.Plant physiology · 2019Article
- Na⁺-Dependent High-Affinity Nitrate, Phosphate and Amino Acids Transport in Leaf Cells of the SeagrassInternational journal of molecular sciences · 2018Article
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Putative phosphate transporters have been identified in a barley (Hordeum vulgare L.) genomic library by their homology to known phosphate transporters from dicot species. The genes designated HORvu;Pht1;1 and HORvu;Pht1;6 encode proteins of 521 and 535 amino acids respectively with 12 predicted membrane-spanning domains and other motifs common to the Phtl family of phosphate transporters. HORvu;Pht1;1 is expressed exclusively in roots and is strongly induced by phosphate deprivation. HORvu;Pht1;6 is expressed in the aerial parts of the plant with strongest expression in old leaves and flag leaves. In situ hybridization showed that HORvu;Pht1;6 is expressed in the phloem of vascular bundles in leaves and ears. In order to study the biochemical properties of HORvu;Pht1;1 and HORvu;Pht1;6, the genes were expressed in transgenic rice (Oryza sativa L.) plants under the control of the rice actin promoter and suspension cell cultures were generated. Cells derived from transgenic plants were able to take up phosphate at a much higher rate than control cells, demonstrating that both genes encode functional phosphate transporters. The estimated Km for phosphate for cells expressing HORvu;Pht1;1 was 9.06 +/- 0.82 microM, which is characteristic of a high-affinity transporter. The rate of phosphate uptake decreased with increasing pH, suggesting that HORvu;Pht1;1 operates as a H+/H2PO4(-) symporter. In contrast, the estimated Km for phosphate for cells expressing HORvu;Pht1;6 was 385 +/- 61 microM, which is characteristic of a low-affinity transporter. Taken together, the results suggest that HORvu;Pht1;1 functions in uptake of phosphate at the root surface, while HORvu;Pht1;6 probably functions in remobilization of stored phosphate from leaves.
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