ArticleHistochemistry and cell biology2004
Early signs of lung fibrosis after in vitro treatment of rat lung slices with CdCl2 and TGF-beta1.
Article in Histochemistry and cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 63 citations in OpenAlex.
- Assessment of Imatinib Anti-Remodeling Activity on a Human Precision Cut Lung Slices Model.International journal of molecular sciences · 2024Article
- Evaluation of an ex vivo fibrogenesis model using human lung slices prepared from small tissues.European journal of medical research · 2023Article
- The Challenge of Long-Term Cultivation of Human Precision-Cut Lung Slices.The American journal of pathology · 2022Article
- Applications and Approaches for Three-Dimensional Precision-Cut Lung Slices. Disease Modeling and Drug Discovery.American journal of respiratory cell and molecular biology · 2020Review
- Rho-Kinase 1/2 Inhibition Prevents Transforming Growth Factor-β-Induced Effects on Pulmonary Remodeling and Repair.Frontiers in pharmacology · 2020Article
- Use of precision cut lung slices as a translational model for the study of lung biology.Respiratory research · 2019Review
- P2X7R: independent modulation of aquaporin 5 expression in CdClHistochemistry and cell biology · 2018Article
- In focus in HCB.Histochemistry and cell biology · 2018Article
- Airway remodeling disease: primary human structural cells and phenotypic and pathway assays to identify targets with potential to prevent or reverse remodeling.Journal of experimental pharmacology · 2018Review
- Potential contribution of alveolar epithelial type I cells to pulmonary fibrosis.Bioscience reports · 2017Review
- Assessment of long-term cultivated human precision-cut lung slices as an ex vivo system for evaluation of chronic cytotoxicity and functionality.Journal of occupational medicine and toxicology (London, England) · 2017Article
- Indoxyl Sulfate as a Mediator Involved in Dysregulation of Pulmonary Aquaporin-5 in Acute Lung Injury Caused by Acute Kidney Injury.International journal of molecular sciences · 2016Article
- Caveolin proteins: a molecular insight into disease.Frontiers of medicine · 2016Review
- Increased AGE-RAGE ratio in idiopathic pulmonary fibrosis.Respiratory research · 2016Article
- Establishment of a Rat Adjuvant Arthritis-Interstitial Lung Disease Model.BioMed research international · 2016Article
- Article
- Distribution of caveolin in the muscle spindles of human skeletal muscle.Journal of anatomy · 2014Article
- Comparison of airway responses in sheep of different age in precision-cut lung slices (PCLS).PloS one · 2014Article
- Effect of P2X7 receptor knockout on AQP-5 expression of type I alveolar epithelial cells.PloS one · 2014Article
- Receptor for advanced glycation end products and its involvement in inflammatory diseases.International journal of inflammation · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Precision-cut rat lung slices have been employed in combination with an extensive immunohistochemistry of paraffin-embedded slices for monitoring of early pathohistological changes after exposure to CdCl(2)/TGF-beta(1). Three days of CdCl(2) exposure in combination with TGF-beta(1) seem to be sufficient to induce lung injury with alterations similar to changes observed in early lung fibrogenesis: (1) extracellular matrix accumulation and myofibroblast transdifferentiation (Sirius red staining, collagen type IV, alpha-smooth muscle actin), (2) type I cell injury with loss of type I cell antigens (T1alpha antigen, aquaporin-5, RAGE), (3) increased apoptosis of pulmonary cells (active caspase-3, vimentin cleavage product V1 of caspase-9), and (4) activation of microvascular endothelial cells (podocalyxin, caveolin-1). Western blot analysis confirmed the increasing amount of alpha-smooth muscle actin, the loss of T1alpha antigen, and the increase in caveolin-1 immunoreactivity. The explant culture using CdCl(2)/TGF-beta(1) provides a suitable tool for the study of other factors involved in pulmonary pathology including transcription factors, cytokines, and other metabolites involved in early stages of fibrogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.