ArticleJournal of biomolecular NMR2004
Application of correlated residual dipolar couplings to the determination of the molecular alignment tensor magnitude of oriented proteins and nucleic acids.
Article in Journal of biomolecular NMR, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Solution Domain Dynamics of Monomeric SARS-CoV‑2 Main Protease Revealed by Optimized NMR Residual Dipolar Coupling Measurements.ACS physical chemistry Au · 2026Article
- Alpha-helices as alignment reporters in residual dipolar coupling analysis of proteins.Journal of biomolecular NMR · 2025Article
- Unequivocal structure confirmation of a breitfussin analog by anisotropic NMR measurements.Chemical science · 2020Article
- Maximizing accuracy of RNA structure in refinement against residual dipolar couplings.Journal of biomolecular NMR · 2019Article
- Engineering encodable lanthanide-binding tags into loop regions of proteins.Journal of the American Chemical Society · 2011Article
- NMR studies of a channel protein without membranes: structure and dynamics of water-solubilized KcsA.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Liquid-crystal NMR structure of HIV TAR RNA bound to its SELEX RNA aptamer reveals the origins of the high stability of the complex.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Solution structure of Ca2+-free rat beta-parvalbumin (oncomodulin).Protein science : a publication of the Protein Society · 2007Article
- Top-down approach in protein RDC data analysis: de novo estimation of the alignment tensor.Journal of biomolecular NMR · 2007Article
- HIFI-C: a robust and fast method for determining NMR couplings from adaptive 3D to 2D projections.Journal of biomolecular NMR · 2007Article
- Prediction of molecular alignment of nucleic acids in aligned media.Journal of biomolecular NMR · 2006Article
- iDC: A comprehensive toolkit for the analysis of residual dipolar couplings for macromolecular structure determination.Journal of biomolecular NMR · 2006Article
- Rapid and accurate structure determination of coiled-coil domains using NMR dipolar couplings: application to cGMP-dependent protein kinase Ialpha.Protein science : a publication of the Protein Society · 2005Article
- Structure of calmodulin complexed with an olfactory CNG channel fragment and role of the central linker: residual dipolar couplings to evaluate calmodulin binding modes outside the kinase family.Journal of biomolecular NMR · 2005Article
- Pre-organized structure of viral DNA at the binding-processing site of HIV-1 integrase.Nucleic acids research · 2005Article
- Resolution-optimized NMR measurement of (1)D(CH), (1)D(CC) and (2)D(CH) residual dipolar couplings in nucleic acid bases.Journal of biomolecular NMR · 2004Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
Abstract
Residual dipolar couplings (RDC) between nuclear spins in partially aligned samples offer unique insights into biomacromolecular structure and dynamics. To fully benefit from the RDC data, accurate knowledge of the magnitude ( D (a)) and rhombicity ( R ) of the molecular alignment tensor, A, is important. An extended histogram method (EHM) is presented which extracts these parameters more effectively from dipolar coupling data. The method exploits the correlated nature of RDCs for structural elements of planar geometry, such as the one-bond (13)C'(i)-(13)C(i)(alpha), (13)C'(i)-(15)N(i+1), and (15)N(i+1)-(1)H(N)(i+1) couplings in peptide bonds of proteins, or suitably chosen combinations of (1) D (C1'H1'), (1) D (C2'H2'), (1) D (C1'C2'), (2) D (C2'H1'), (2) D (C1'H2'), and (3) D (H1'H2') couplings in nucleic acids, to generate an arbitrarily large number of synthetic RDCs. These synthetic couplings result in substantially improved histograms and resulting values of D (a) and R, compared with histograms generated solely from the original sets of correlated RDCs, particularly when the number of planar fragments for which couplings are available is small. An alternative method, complementary to the EHM, is also described, which uses a systematic grid search procedure, based on least-squares fitting of sets of correlated RDCs to structural elements of known geometry, and provides an unambiguous lower limit for the degree of molecular alignment.
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