Evidence map›Paper›PMID 14752044›Full record

ArticleNucleic acids research2004

Oligonucleotide-directed site-specific integration of high complexity libraries into ssDNA templates.

M B Hale, G P Nolan, R Wolkowicz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

M B HaleDepartment of Molecular Pharmacology, School of Medicine, Stanford University, Stanford, CA 94305, USA.
G P Nolan
R Wolkowicz

Funding

NF-KB P50 AND BCL-3 AS COREGULATORS OF HIV AND BCL-3R01AI035304 · NIAID · STANFORD UNIVERSITY · PI NOLAN, GARRY P · 1994 to 2004
$1.5M
RETROVIRAL MEDIATED GENE THERAPY OF RHEUMATOID ARTHRITISN01AR062227 · NIAMS · STANFORD UNIVERSITY · PI FATHMAN, CHARLES GARRISON · 1996 to 2000
$528k
SYNOVIAL TARGETING OF INDUCIBLE GENESR01AR044565 · NIAMS · STANFORD UNIVERSITY · PI NOLAN, GARRY P · 1996 to 2000
$238k
NHLBI PROTEOMICS INITIATIVE-N01HV28183N01HV028183 · HV · STANFORD UNIVERSITY · PI NOLAN, GARRY P · 2002 to 2006
–
USE OF ALTERED PEPTIDE LIGANDS IN THE TREATMENT OF DIABETESP01AI039646 · NIAID · STANFORD UNIVERSITY · PI FATHMAN, CHARLES GARRISON · 1996 to 1999
–
PLATELET INTERACTIONS WITH FIBRINOGEN AND CALCIUMR01HL028183 · NHLBI · STATE UNIVERSITY NEW YORK STONY BROOK · PI PEERSCHKE, ELLINOR I · 1985 to 1992
–
NHLBI NIH HHS N01 HV028183NHLBI NIH HHS N01-HV-28183INIAID NIH HHS AI35304NIAID NIH HHS P01-AI39646NIAID NIH HHS R01 AI035304NIAMS NIH HHS AR44565NIAMS NIH HHS N01-AR-6-2227NIH HHS A1/GF41520-01PHS HHS 2HPA412PHS HHS 2HPZ713
6 · The paper itself

Abstract

We present an approach that generates an oligomer-based library with minimal need for restriction site modification of sequences in the target vector. The technique has the advantage that it can be applied for generating peptide aptamer libraries at sites within proteins without the need for introducing flanking enzyme sites. As an example we present a phagemid retroviral shuttle vector that can be used to achieve stable expression of the library in mammalian cells for the purpose of screening for peptides with desired biological activity.

Indexed as

Gene LibraryBase SequenceBinding SitesCell LineCell SurvivalCloning, MolecularDeoxyribonucleases, Type II Site-SpecificDNADNA, Single-StrandedEscherichia coliGenetic VectorsHumansMolecular Sequence DataOligonucleotidesPyrrolidinonesReproducibility of Resultsblasticidin ACTCGAG-specific type II deoxyribonucleasesDeoxyribonucleases, Type II Site-SpecificDNADNA, Single-Strandedendodeoxyribonuclease PaeIOligonucleotidesPyrrolidinones

Identifiers

PMID14752044
PMCPMC373376

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.