ArticlePlant molecular biology2003
Expression and RNA binding properties of the chloroplast ribosomal protein S1 from Chlamydomonas reinhardtii.
Article in Plant molecular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Proteostasis and protein quality control in chloroplasts: mechanisms and novel insights related to protein mislocalization.Journal of experimental botany · 2025Review
- Perturbation of protein homeostasis brings plastids at the crossroad between repair and dismantling.PLoS genetics · 2023Article
- BE3 is the major branching enzyme isoform required for amylopectin synthesis in CFrontiers in plant science · 2023Article
- Albino seedling lethality 4; Chloroplast 30S Ribosomal Protein S1 is Required for Chloroplast Ribosome Biogenesis and Early Chloroplast Development in Rice.Rice (New York, N.Y.) · 2021Article
- EDS1-interacting J protein 1 is an essential negative regulator of plant innate immunity in Arabidopsis.The Plant cell · 2021Article
- Deletion of BSG1 in Chlamydomonas reinhardtii leads to abnormal starch granule size and morphology.Scientific reports · 2019Article
- Unique localization of the plastid-specific ribosomal proteins in the chloroplast ribosome small subunit provides mechanistic insights into the chloroplastic translation.Nucleic acids research · 2017Article
- The complete structure of the chloroplast 70S ribosome in complex with translation factor pY.The EMBO journal · 2017Article
- GUN1, a Jack-Of-All-Trades in Chloroplast Protein Homeostasis and Signaling.Frontiers in plant science · 2016Review
- A forward genetic approach in Chlamydomonas reinhardtii as a strategy for exploring starch catabolism.PloS one · 2013Article
- Downregulation of chloroplast RPS1 negatively modulates nuclear heat-responsive expression of HsfA2 and its target genes in Arabidopsis.PLoS genetics · 2012Article
- Article
- ATAB2 is a novel factor in the signalling pathway of light-controlled synthesis of photosystem proteins.The EMBO journal · 2006Article
- Rrp5p, a trans-acting factor in yeast ribosome biogenesis, is an RNA-binding protein with a pronounced preference for U-rich sequences.RNA (New York, N.Y.) · 2006Article
- A novel multifunctional factor involved in trans-splicing of chloroplast introns in Chlamydomonas.Nucleic acids research · 2006Article
- The FLP proteins act as regulators of chlorophyll synthesis in response to light and plastid signals in Chlamydomonas.Genes & development · 2005Article
- Chloroplast elongation factor ts pro-protein is an evolutionarily conserved fusion with the s1 domain-containing plastid-specific ribosomal protein-7.The Plant cell · 2004Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene encoding the chloroplast ribosomal protein S1 from Chlamydomonas reinhardtii, CreS1, was cloned and the RNA binding properties and the expression patterns were studied. Gel-shift analysis revealed that CreS1 binds AU-rich 5'-untranslated regions (5'-UTR) of chloroplast mRNAs with higher affinity than the corresponding sequence of a GC-rich nuclear transcript. The binding affinity of CreS1 for a mutant form of the psbD 5'-UTR with a deletion of a U-rich stretch that is required for translation decreases 4-fold as compared to the wild-type 5'-UTR. Our results suggest that CreS1 protein interacts with U-rich sequences. Most of CreS1 is bound to high-molecular-weight complexes which co-migrate with the 30S small ribosomal subunit, and only a small fraction of CreS1 exists in its free form. CreS1 is localized mainly to the chloroplast stroma albeit a significant fraction is associated with chloroplast membranes. The results suggest that most of CreS1 is associated with the 30S ribosomal subunit throughout the translation process. Upon a shift of cells from the dark to the light, the mRNA levels of CreS1 and Psrp-7, both components of the 30S ribosomal subunit, increase transiently and return to the dark levels after 8 h. However, during this dark-to-light transition the levels of CreS1 and of other components of the 30S subunit remain the same suggesting that either protein synthesis or degradation is regulated. The possible implications of these findings are discussed.
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