ArticlePlant molecular biology2003
Characterization of the targeting signal of dual-targeted pea glutathione reductase.
Article in Plant molecular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 83 citations in OpenAlex.
- Reliability of plastid and mitochondrial localisation prediction declines rapidly with the evolutionary distance to the training set increasing.PLoS computational biology · 2024Article
- The Glutathione System: A Journey from Cyanobacteria to Higher Eukaryotes.Antioxidants (Basel, Switzerland) · 2023Review
- Glutathione Metabolism in Plants under Stress: Beyond Reactive Oxygen Species Detoxification.Metabolites · 2021Review
- Go your own way: membrane-targeting sequences.Plant physiology · 2021Article
- PtomtAPX, a mitochondrial ascorbate peroxidase, plays an important role in maintaining the redox balance of Populus tomentosa Carr.Scientific reports · 2019Article
- Vitamin C in Plants: From Functions to Biofortification.Antioxidants (Basel, Switzerland) · 2019Review
- Article
- The dual targeting ability of type II NAD(P)H dehydrogenases arose early in land plant evolution.BMC plant biology · 2013Article
- Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants.Frontiers in plant science · 2013Article
- Rice MADS3 regulates ROS homeostasis during late anther development.The Plant cell · 2011Article
- The NADPH-dependent thioredoxin system constitutes a functional backup for cytosolic glutathione reductase in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Exploring the function-location nexus: using multiple lines of evidence in defining the subcellular location of plant proteins.The Plant cell · 2009Article
- Dual targeting of organellar seryl-tRNA synthetase to maize mitochondria and chloroplasts.Plant cell reports · 2008Article
- Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active beta-amylases in Arabidopsis chloroplasts.The Plant cell · 2008Article
- Mitochondrial biogenesis and function in Arabidopsis.The arabidopsis book · 2008Article
- Functional definition of outer membrane proteins involved in preprotein import into mitochondria.The Plant cell · 2007Article
- Dual targeting of antioxidant and metabolic enzymes to the mitochondrion and the apicoplast of Toxoplasma gondii.PLoS pathogens · 2007Article
- Nine 3-ketoacyl-CoA thiolases (KATs) and acetoacetyl-CoA thiolases (ACATs) encoded by five genes in Arabidopsis thaliana are targeted either to peroxisomes or cytosol but not to mitochondria.Plant molecular biology · 2007Article
- Characterization of the preprotein and amino acid transporter gene family in Arabidopsis.Plant physiology · 2007Article
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the dual targeting signal of pea glutathione reductase (GR) that had been previously shown to be capable of targeting the passenger protein phosphinothricin acetyl transferase to mitochondria and chloroplasts in vivo. We confirmed that GR was imported into mitochondria and chloroplasts in vitro. Rupture of the outer mitochondrial membrane after the import assay indicated that GR was imported into both the intermembrane space and the matrix. Changing positive and hydrophobic residues in the targeting signal we investigated if dual targeting of GR was due to an overlapping or separate signal. Overall single mutations had a greater effect on mitochondrial import compared to chloroplasts, especially those on positive residues. Precursors containing both positive and hydrophobic residue mutations (double mutants) indicated that there might be some redundancy in targeting information for chloroplastic import as double mutants had a greater effect than predicted from the single mutants. Fusion of the targeting signal to the green fluorescent protein (GFP) followed by transient transformation indicated that this signal was only capable of targeting this passenger protein to plastids. Additionally, fusion of the complete coding sequence of GR to GFP also resulted in an exclusive chloroplastic localization. Mutations in the targeting signal that reduced import into plastids in vitro also displayed altered patterns of GFP localizations in vivo. These results indicate that some residues in the signal for dual localisation of GR play a role in both mitochondrial and chloroplastic import, and thus the signal is overlapping.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.