ArticleEMBO reports2004
Analysis of Scc1-deficient cells defines a key metaphase role of vertebrate cohesin in linking sister kinetochores.
Article in EMBO reports, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 70 citations in OpenAlex.
- Article
- A tribute to 40 years of CENP-A & centromere pioneer Bill Earnshaw.Chromosoma · 2025Article
- Mapping separase-mediated cleavage in situ.NAR genomics and bioinformatics · 2022Article
- Mitotic chromosomes.Seminars in cell & developmental biology · 2021Review
- Review
- A Topology-Centric View on Mitotic Chromosome Architecture.International journal of molecular sciences · 2017Review
- Article
- The SUMO deconjugating peptidase Smt4 contributes to the mechanism required for transition from sister chromatid arm cohesion to sister chromatid pericentromere separation.Cell cycle (Georgetown, Tex.) · 2015Article
- Article
- Cohesin removal precedes topoisomerase IIα-dependent decatenation at centromeres in male mammalian meiosis II.Chromosoma · 2014Article
- Direct monitoring of the strand passage reaction of DNA topoisomerase II triggers checkpoint activation.PLoS genetics · 2013Article
- Mitotic chromosomes are compacted laterally by KIF4 and condensin and axially by topoisomerase IIα.The Journal of cell biology · 2012Article
- Cohesin-SA1 deficiency drives aneuploidy and tumourigenesis in mice due to impaired replication of telomeres.The EMBO journal · 2012Article
- Cohesin-independent segregation of sister chromatids in budding yeast.Molecular biology of the cell · 2012Article
- Spatial exclusivity combined with positive and negative selection of phosphorylation motifs is the basis for context-dependent mitotic signaling.Science signaling · 2011Article
- Building mitotic chromosomes.Current opinion in cell biology · 2011Review
- Kinetochore-microtubule interactions: steps towards bi-orientation.The EMBO journal · 2010Review
- PICH and cotargeted Plk1 coordinately maintain prometaphase chromosome arm architecture.Molecular biology of the cell · 2010Article
- Studies of haspin-depleted cells reveal that spindle-pole integrity in mitosis requires chromosome cohesion.Journal of cell science · 2009Article
- Clearing the way for mitosis: is cohesin a target?Nature reviews. Molecular cell biology · 2009Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
Abstract
Cleavage of the cohesin subunit Scc1p/Mcd1p/Rad21 permits sister chromatid separation and is considered to trigger anaphase onset. It has also been suggested that the cohesin complex is essential for chromosome condensation and for assembling fully functional kinetochores. Here, we used vertebrate cells conditionally deficient in Scc1 to probe cohesin function in mitosis. Cells lacking cohesin arrest in prometaphase, with many chromosomes failing to align at a metaphase plate and high levels of the spindle assembly checkpoint protein, BubR1, at all kinetochores. We show that the structural integrity of chromosomes is normal in the absence of Scc1. Furthermore, specific inhibition of topoisomerase II, which is required for decatenation of replicated chromosomes, can bypass the cohesin requirement for metaphase chromosome alignment and spindle checkpoint silencing. Since the kinetochore effects of Scc1 deficiency can be compensated for by topoisomerase II inhibition, we conclude that Scc1 is not absolutely required for kinetochore assembly or function, and that its principal role in allowing the onset of anaphase is the establishment of sufficient inter-sister tension to allow biorientation.
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Registered trials
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