ArticleMolecular and cellular biology2004
Activation of H-Ras in the endoplasmic reticulum by the RasGRF family guanine nucleotide exchange factors.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 96 citations in OpenAlex.
- CD59-Regulated Ras Compartmentalization Orchestrates Antitumor T-cell Immunity.Cancer immunology research · 2022Article
- RASGRF1 Fusions Activate Oncogenic RAS Signaling and Confer Sensitivity to MEK Inhibition.Clinical cancer research : an official journal of the American Association for Cancer Research · 2022Article
- Ras Isoforms from Lab Benches to Lives-What Are We Missing and How Far Are We?International journal of molecular sciences · 2021Review
- The Ins and Outs of RAS Effector Complexes.Biomolecules · 2021Review
- Palmitoylation as a Key Regulator of Ras Localization and Function.Frontiers in molecular biosciences · 2021Review
- Characterisation of HRas local signal transduction networks using engineered site-specific exchange factors.Small GTPases · 2020Article
- Ras and Rap1: A tale of two GTPases.Seminars in cancer biology · 2019Review
- RAS at the Golgi antagonizes malignant transformation through PTPRκ-mediated inhibition of ERK activation.Nature communications · 2018Article
- RasGRF Couples Nox4-Dependent Endoplasmic Reticulum Signaling to Ras.Arteriosclerosis, thrombosis, and vascular biology · 2017Article
- Modeling of RAS complexes supports roles in cancer for less studied partners.BMC biophysics · 2017Article
- PGA1-induced apoptosis involves specific activation of H-Ras and N-Ras in cellular endomembranes.Cell death & disease · 2016Article
- Defined spatiotemporal features of RAS-ERK signals dictate cell fate in MCF-7 mammary epithelial cells.Molecular biology of the cell · 2016Article
- Endocytosis separates EGF receptors from endogenous fluorescently labeled HRas and diminishes receptor signaling to MAP kinases in endosomes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Comparative proteomic analysis of compartmentalised Ras signalling.Scientific reports · 2015Article
- PAQR3 regulates Golgi vesicle fission and transport via the Gβγ-PKD signaling pathway.Cellular signalling · 2015Article
- Spatial control of Cdc42 signalling by a GM130-RasGRF complex regulates polarity and tumorigenesis.Nature communications · 2014Article
- The effect of alendronate on proteome of hepatocellular carcinoma cell lines.International journal of proteomics · 2014Article
- The differential effects of wild-type and mutated K-Ras on MST2 signaling are determined by K-Ras activation kinetics.Molecular and cellular biology · 2013Article
- Activated Ras as a Therapeutic Target: Constraints on Directly Targeting Ras Isoforms and Wild-Type versus Mutated Proteins.ISRN oncology · 2013Review
- RasGRF1 regulates proliferation and metastatic behavior of human alveolar rhabdomyosarcomas.International journal of oncology · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent findings indicate that in addition to its location in the peripheral plasma membrane, H-Ras is found in endomembranes like the endoplasmic reticulum and the Golgi complex. In these locations H-Ras is functional and can efficiently engage downstream effectors, but little is known about how its activation is regulated in these environments. Here we show that the RasGRF family exchange factors, both endogenous and ectopically expressed, are present in the endoplasmic reticulum but not in the Golgi complex. With the aid of H-Ras constructs specifically tethered to the plasma membrane, endoplasmic reticulum, and Golgi complex, we demonstrate that RasGRF1 and RasGRF2 can activate plasma membrane and reticular, but not Golgi-associated, H-Ras. We also show that RasGRF DH domain is required for the activation of H-Ras in the endoplasmic reticulum but not in the plasma membrane. Furthermore, we demonstrate that RasGRF mediation favors the activation of reticular H-Ras by lysophosphatidic acid treatment whereas plasma membrane H-Ras is made more responsive to stimulation by ionomycin. Overall, our results provide the initial insights into the regulation of H-Ras activation in the endoplasmic reticulum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.