ArticleBiophysical journal2004
Micromechanical tests of adhesion dynamics between neutrophils and immobilized ICAM-1.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 56 citations in OpenAlex.
- Extending applications of AFM to fluidic AFM in single living cell studies.Journal of cellular physiology · 2022Review
- A Pillar-Free Diffusion Device for Studying Chemotaxis on Supported Lipid Bilayers.Micromachines · 2021Article
- Visualization of integrin molecules by fluorescence imaging and techniques.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2021Article
- Endothelial Glycocalyx Layer Properties and Its Ability to Limit Leukocyte Adhesion.Biophysical journal · 2020Article
- Mechanistic Understanding of Single-Cell Behavior is Essential for Transformative Advances in Biomedicine.The Yale journal of biology and medicine · 2018Review
- α6 Integrin (α6International journal of molecular sciences · 2017Article
- Immobilized IL-8 Triggers Phagocytosis and Dynamic Changes in Membrane Microtopology in Human Neutrophils.Annals of biomedical engineering · 2015Article
- Effect of Storage Temperature on Key Functions of Cultured Retinal Pigment Epithelial Cells.Journal of ophthalmology · 2015Article
- Ligand-mediated friction determines morphodynamics of spreading T cells.Biophysical journal · 2014Article
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- Effects of wall shear stress and its gradient on tumor cell adhesion in curved microvessels.Biomechanics and modeling in mechanobiology · 2012Article
- Determining beta2-integrin and intercellular adhesion molecule 1 binding kinetics in tumor cell adhesion to leukocytes and endothelial cells by a gas-driven micropipette assay.The Journal of biological chemistry · 2011Article
- Role of glucosyltransferase B in interactions of Candida albicans with Streptococcus mutans and with an experimental pellicle on hydroxyapatite surfaces.Applied and environmental microbiology · 2011Article
- Temperature modulation of integrin-mediated cell adhesion.Biophysical journal · 2010Article
- Tunable leuko-polymersomes that adhere specifically to inflammatory markers.Langmuir : the ACS journal of surfaces and colloids · 2010Article
- Signaling and Dynamics of Activation of LFA-1 and Mac-1 by Immobilized IL-8.Cellular and molecular bioengineering · 2010Article
- Molecular accessibility in relation to cell surface topography and compression against a flat substrate.Biophysical journal · 2009Article
- Adhesion between human neutrophils and immobilized endothelial ligand vascular cell adhesion molecule 1: divalent ion effects.Biophysical journal · 2009Article
- Active site formation, not bond kinetics, limits adhesion rate between human neutrophils and immobilized vascular cell adhesion molecule 1.Biophysical journal · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Strong, integrin-mediated adhesion of neutrophils to endothelium during inflammation is a dynamic process, requiring a conformational change in the integrin molecule to increase its affinity for its endothelial counterreceptors. To avoid general activation of the cell, Mg(2+) was used to induce the high-affinity integrin conformation, and micromechanical methods were used to determine adhesion probability to beads coated with the endothelial ligand ICAM-1. Neutrophils in Mg(2+) bind to the beads with much greater frequency and strength than in the presence of Ca(2+). An increase in adhesion strength and frequency was observed with both increasing temperature and contact duration (from 2 s to 1 min, 21 or 37 degrees C). The dependence of adhesion probability on contact time or receptor density yielded estimates of the effective reverse rate constant, k(r), and the equilibrium association constant, K(a), for binding of neutrophils to ICAM-1 coated surfaces in Mg(2+): k(r) approximately 0.7 s(-1) and the product K(a)rho(c) approximately 2.4 x 10(-4), where rho(c) is the density of integrin on the cell surface.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.