ArticleBiophysical journal2004
Patterning, prestress, and peeling dynamics of myocytes.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 54 citations in OpenAlex.
- Cytoskeletal prestress homeostasis is a biological principle that governs living cell structure and function.Mechanobiology in medicine · 2026Review
- Unravelling hagfish slime.Journal of the Royal Society, Interface · 2019Article
- Hydrogel biomaterials and their therapeutic potential for muscle injuries and muscular dystrophies.Journal of the Royal Society, Interface · 2018Review
- Cellular Biomechanics in Drug Screening and Evaluation: Mechanopharmacology.Trends in pharmacological sciences · 2016Review
- Electrical and mechanical stimulation of cardiac cells and tissue constructs.Advanced drug delivery reviews · 2016Review
- The cytoskeleton regulates cell attachment strength.Biophysical journal · 2015Article
- Crosslinking of collagen scaffolds promotes blood and lymphatic vascular stability.Journal of biomedical materials research. Part A · 2014Article
- Biofilm and siderophore effects on secondary waste water disinfection.Current microbiology · 2011Article
- Optimal matrix rigidity for stress fiber polarization in stem cells.Nature physics · 2010Article
- Detecting cell-adhesive sites in extracellular matrix using force spectroscopy mapping.Journal of physics. Condensed matter : an Institute of Physics journal · 2010Article
- Cell shape, spreading symmetry and the polarization of stress-fibers in cells.Journal of physics. Condensed matter : an Institute of Physics journal · 2010Article
- A novel mode of cell detachment from fibrillar fibronectin matrix under shear.Journal of cell science · 2009Article
- A novel functional assessment of the differentiation of micropatterned muscle cells.Journal of biomechanics · 2008Article
- Adhesion of single bacterial cells in the micronewton range.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Long-term microfluidic cultures of myotube microarrays for high-throughput focal stimulation.Nature protocols · 2006Article
- Cholesterol depletion increases membrane stiffness of aortic endothelial cells.Biophysical journal · 2004Article
- Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments.The Journal of cell biology · 2004Article
- Upregulation of paxillin and focal adhesion signaling follows Dystroglycan Complex deletions and promotes a hypertensive state of differentiation.European journal of cell biologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As typical anchorage-dependent cells myocytes must balance contractility against adequate adhesion. Skeletal myotubes grown as isolated strips from myoblasts on micropatterned glass exhibited spontaneous peeling after one end of the myotube was mechanically detached. Such results indicate the development of a prestress in the cells. To assess this prestress and study the dynamic adhesion strength of single myocytes, the shear stress of fluid aspirated into a large-bore micropipette was then used to forcibly peel myotubes. The velocity at which cells peeled from the surface, V(peel), was measured as a continuously increasing function of the imposed tension, T(peel), which ranges from approximately 0 to 50 nN/ micro m. For each cell, peeling proved highly heterogeneous, with V(peel) fluctuating between 0 micro m/s ( approximately 80% of time) and approximately 10 micro m/s. Parallel studies of smooth muscle cells expressing GFP-paxillin also exhibited a discontinuous peeling in which focal adhesions fractured above sites of strong attachment (when pressure peeled using a small-bore pipette). The peeling approaches described here lend insight into the contractile-adhesion balance and can be used to study the real-time dynamics of stressed adhesions through both physical detection and the use of GFP markers; the methods should prove useful in comparing normal versus dystrophic muscle cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.