ArticleCurrent genetics2004
A one-step method to convert vectors into binary vectors suited for Agrobacterium-mediated transformation.
Article in Current genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 89 citations in OpenAlex.
- The Effector SIX8 Contributes to Virulence of Fusarium oxysporum f. sp. lactucae Race 4 on Lettuce.Molecular plant pathology · 2026Article
- Horizontal Chromosome Transfer between Pathogenic and Non-pathogenic Fusarium oxysporum Strains Isolated from Cabbage.Microbes and environments · 2026Article
- Divergent ECC1 effector homologs modulate host-specific virulence in cucurbit-infectingFrontiers in cellular and infection microbiology · 2025Article
- Application and Technical Challenges in Design, Cloning, and Transfer of Large DNA.Bioengineering (Basel, Switzerland) · 2023Review
- FungalBraid 2.0: expanding the synthetic biology toolbox for the biotechnological exploitation of filamentous fungi.Frontiers in bioengineering and biotechnology · 2023Article
- A primary cell wall cellulose-dependent defense mechanism against vascular pathogens revealed by time-resolved dual transcriptomics.BMC biology · 2021Article
- A pair of effectors encoded on a conditionally dispensable chromosome of Fusarium oxysporum suppress host-specific immunity.Communications biology · 2021Article
- Comparative genomics of Fusarium oxysporum f. sp. melonis strains reveals nine lineages and a new sequence type of AvrFom2.Environmental microbiology · 2021Article
- A single gene in Fusarium oxysporum limits host range.Molecular plant pathology · 2021Article
- Partial pathogenicity chromosomes in Fusarium oxysporum are sufficient to cause disease and can be horizontally transferred.Environmental microbiology · 2020Article
- The root-invading pathogen Fusarium oxysporum targets pattern-triggered immunity using both cytoplasmic and apoplastic effectors.The New phytologist · 2020Article
- Related mobile pathogenicity chromosomes in Fusarium oxysporum determine host range on cucurbits.Molecular plant pathology · 2020Article
- Pathogen-induced pH changes regulate the growth-defense balance in plants.The EMBO journal · 2019Article
- Advances in linking polyketides and non-ribosomal peptides to their biosynthetic gene clusters in Fusarium.Current genetics · 2019Review
- A mobile pathogenicity chromosome in Fusarium oxysporum for infection of multiple cucurbit species.Scientific reports · 2017Article
- Fungal phytopathogens encode functional homologues of plant rapid alkalinization factor (RALF) peptides.Molecular plant pathology · 2017Article
- A silver bullet in a golden age of functional genomics: the impact ofFungal biology and biotechnology · 2017Review
- Transcription Factors Encoded on Core and Accessory Chromosomes of Fusarium oxysporum Induce Expression of Effector Genes.PLoS genetics · 2016Article
- Comparative genomics of Fusarium oxysporum f. sp. melonis reveals the secreted protein recognized by the Fom-2 resistance gene in melon.The New phytologist · 2016Article
- Ecologically driven competence for exogenous DNA uptake in yeast.Current microbiology · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial artificial chromosomes (BACs) are widely used for the construction of physical maps, positional-cloning and whole-genome sequencing strategies. Unfortunately, their use for functional genomics is limited, as currently there is no efficient method to use BACs directly for complementation. We describe a novel strategy for one-step conversion of any BAC into a binary BAC (BIBAC). Using Agrobacterium tumefaciens, these BIBACs can be efficiently transformed to virtually all organisms, including plants, fungi, yeasts and human cells. As the strategy is based on in vivo recombineering and does not depend on restriction sites, it is applicable to any vector. To show the feasibility of the method five BACs, containing 0-75 kb of fungal DNA, were converted into BIBACs. These were subsequently transformed to the plant pathogenic fungus Fusarium oxysporum f.sp. lycopersici and to Aspergillus awamori, a filamentous fungus often used for large-scale protein production. Molecular characterisation of the transformants showed that the BIBACs were efficiently transferred to the fungi and stably integrated into their genomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.