ArticleProceedings of the National Academy of Sciences of the United States of America2004
Folate synthesis in plants: the p-aminobenzoate branch is initiated by a bifunctional PabA-PabB protein that is targeted to plastids.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 123 citations in OpenAlex.
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- Multi-omic analysis of the extension of broccoli quality during storage by folic acid.Journal of advanced research · 2024Article
- The shikimate pathway: gateway to metabolic diversity.Natural product reports · 2024Review
- Transcriptional Profiling Reveals the Wheat Defences against Fusarium Head Blight Disease Regulated by a NAC Transcription Factor.Plants (Basel, Switzerland) · 2023Article
- Radiosynthesis, Preclinical, and Clinical Positron Emission Tomography Studies of Carbon-11 Labeled Endogenous and Natural Exogenous Compounds.Chemical reviews · 2023Review
- Genome Mining and Metabolomics Unveil Pseudonochelin: A Siderophore Containing 5-Aminosalicylate from a Marine-DerivedOrganic letters · 2022Article
- Comparative Transcriptome Analysis Reveals Mechanisms of Folate Accumulation in Maize Grains.International journal of molecular sciences · 2022Article
- A genome-wide association study of folates in sweet corn kernels.Frontiers in plant science · 2022Article
- Red light enhances folate accumulation in wheat seedlings.Journal of Zhejiang University. Science. B · 2021Article
- Antioxidants in Potatoes: A Functional View on One of the Major Food Crops Worldwide.Molecules (Basel, Switzerland) · 2021Review
- Bioprospecting Fluorescent Plant Growth Regulators from Arabidopsis to Vegetable Crops.International journal of molecular sciences · 2021Article
- Mutations in themicroPublication biology · 2021Article
- Sulfamethoxazole-Altered Transcriptomein Green AlgaFrontiers in microbiology · 2021Article
- Article
- Strategies for the production of biochemicals in bioenergy crops.Biotechnology for biofuels · 2020Review
- Evolution of folate biosynthesis and metabolism across algae and land plant lineages.Scientific reports · 2019Article
- Improved folate accumulation in genetically modified maize and wheat.Journal of experimental botany · 2019Article
- Engineering of Biosynthesis Pathway and NADPH Supply for Improved L-5-Methyltetrahydrofolate Production byJournal of microbiology and biotechnology · 2019Article
- From Central to Specialized Metabolism: An Overview of Some Secondary Compounds Derived From the Primary Metabolism for Their Role in Conferring Nutritional and Organoleptic Characteristics to Fruit.Frontiers in plant science · 2019Review
Corrections and comments
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Authors and funding
11 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is not known how plants synthesize the p-aminobenzoate (PABA) moiety of folates. In Escherichia coli, PABA is made from chorismate in two steps. First, the PabA and PabB proteins interact to catalyze transfer of the amide nitrogen of glutamine to chorismate, forming 4-amino-4-deoxychorismate (ADC). The PabC protein then mediates elimination of pyruvate and aromatization to give PABA. Fungi, actinomycetes, and Plasmodium spp. also synthesize PABA but have proteins comprising fused domains homologous to PabA and PabB. These bipartite proteins are commonly called "PABA synthases," although it is unclear whether they produce PABA or ADC. Genomic approaches identified Arabidopsis and tomato cDNAs encoding bipartite proteins containing fused PabA and PabB domains, plus a putative chloroplast targeting peptide. These cDNAs encode functional enzymes, as demonstrated by complementation of an E. coli pabA pabB double mutant and a yeast PABA-synthase deletant. The partially purified recombinant Arabidopsis protein did not produce PABA unless the E. coli PabC enzyme was added, indicating that it forms ADC, not PABA. The enzyme behaved as a monomer in size-exclusion chromatography and was not inhibited by physiological concentrations of PABA, its glucose ester, or folates. When the putative targeting peptide was fused to GFP and expressed in protoplasts, the fusion protein appeared only in chloroplasts, indicating that PABA synthesis is plastidial. In the pericarp of tomato fruit, the PabA-PabB mRNA level fell drastically as ripening advanced, but there was no fall in total PABA content, which stayed between 0.7 and 2.3 nmol.g(-1) fresh weight.
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