ArticleProceedings of the National Academy of Sciences of the United States of America2004
Method for analyzing signaling networks in complex cellular systems.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Phenotypic screening of the ToxCast chemical library to classify toxic and therapeutic mechanisms.Nature biotechnology · 2014Article
- Regulation of IL-17A production is distinct from IL-17F in a primary human cell co-culture model of T cell-mediated B cell activation.PloS one · 2013Article
- Inferring biochemical reaction pathways: the case of the gemcitabine pharmacokinetics.BMC systems biology · 2012Article
- Secretory protein profiling reveals TNF-α inactivation by selective and promiscuous Sec61 modulators.Chemistry & biology · 2011Article
- Discovery of dual inhibitors of the immune cell PI3Ks p110delta and p110gamma: a prototype for new anti-inflammatory drugs.Chemistry & biology · 2010Article
- Connecting synthetic chemistry decisions to cell and genome biology using small-molecule phenotypic profiling.Current opinion in chemical biology · 2009Review
- Discovering active compounds from mixture of natural products by data mining approach.Medical & biological engineering & computing · 2008Article
- Understanding endothelial cell apoptosis: what can the transcriptome, glycome and proteome reveal?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2007Review
- Predicting protein-protein interactions based only on sequences information.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- An efficient rapid system for profiling the cellular activities of molecular libraries.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Systems biology in drug discovery.Nature biotechnology · 2004Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Now that the human genome has been sequenced, the challenge of assigning function to human genes has become acute. Existing approaches using microarrays or proteomics frequently generate very large volumes of data not directly related to biological function, making interpretation difficult. Here, we describe a technique for integrative systems biology in which: (i) primary cells are cultured under biologically meaningful conditions; (ii) a limited number of biologically meaningful readouts are measured; and (iii) the results obtained under several different conditions are combined for analysis. Studies of human endothelial cells overexpressing different signaling molecules under multiple inflammatory conditions show that this system can capture a remarkable range of functions by a relatively small number of simple measurements. In particular, measurement of seven different protein levels by ELISA under four different conditions is capable of reconstructing pathway associations of 25 different proteins representing four known signaling pathways, implicating additional participants in the NF-kappaBorRAS/mitogen-activated protein kinase pathways and defining additional interactions between these pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.