ArticleThe Journal of experimental medicine2004
Asymmetric division and lineage commitment at the level of hematopoietic stem cells: inference from differentiation in daughter cell and granddaughter cell pairs.
Article in The Journal of experimental medicine, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
75 citing papers in PubMed, 201 citations in OpenAlex.
- Cellular and molecular mechanisms of asymmetric stem cell division in tissue homeostasis.Genes to cells : devoted to molecular & cellular mechanisms · 2024Review
- Early megakaryocyte lineage-committed progenitors in adult mouse bone marrow.Blood science (Baltimore, Md.) · 2024Article
- SON is an essential mCell stem cell · 2023Article
- LinCell proliferation · 2023Article
- Linking cell cycle to hematopoietic stem cell fate decisions.Frontiers in cell and developmental biology · 2023Review
- Murine foetal liver supports limited detectable expansion of life-long haematopoietic progenitors.Nature cell biology · 2022Article
- Arhgef2 regulates mitotic spindle orientation in hematopoietic stem cells and is essential for productive hematopoiesis.Blood advances · 2021Article
- Computational modeling of stem and progenitor cell kinetics identifies plausible hematopoietic lineage hierarchies.iScience · 2021Article
- Research progress on the structure and function of endomucin.Animal models and experimental medicine · 2020Review
- In Vivo Pre-Instructed HSCs Robustly Execute Asymmetric Cell Divisions In Vitro.International journal of molecular sciences · 2020Article
- Towards Mimicking the Fetal Liver Niche: The Influence of Elasticity and Oxygen Tension on Hematopoietic Stem/Progenitor Cells Cultured in 3D Fibrin Hydrogels.International journal of molecular sciences · 2020Article
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- Chromatin regulator Asxl1 loss and Nf1 haploinsufficiency cooperate to accelerate myeloid malignancy.The Journal of clinical investigation · 2018Article
- Restricted cell cycle is essential for clonal evolution and therapeutic resistance of pre-leukemic stem cells.Nature communications · 2018Article
- Dynamics of Bone Marrow VSELs and HSCs in Response to Treatment with Gonadotropin and Steroid Hormones, during Pregnancy and Evidence to Support Their Asymmetric/Symmetric Cell Divisions.Stem cell reviews and reports · 2018Article
- Dissection of Signaling Events Downstream of the c-Mpl Receptor in Murine Hematopoietic Stem Cells Via Motif-Engineered Chimeric Receptors.Stem cell reviews and reports · 2018Article
- HSC Niche Biology and HSC Expansion Ex Vivo.Trends in molecular medicine · 2017Review
- The development of T cells from stem cells in mice and humans.Future science OA · 2017Review
- Dysregulation of haematopoietic stem cell regulatory programs in acute myeloid leukaemia.Journal of molecular medicine (Berlin, Germany) · 2017Review
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How hematopoietic stem cells (HSCs) commit to a particular lineage is unclear. A high degree of HSC purification enabled us to address this issue at the clonal level. Single-cell transplantation studies revealed that 40% of the CD34-/low, c-Kit+, Sca-1+, and lineage marker- (CD34-KSL) cells in adult mouse bone marrow were able, as individual cells, to reconstitute myeloid and B- and T-lymphoid lineages over the long-term. Single-cell culture showed that >40% of CD34-KSL cells could form neutrophil (n)/macrophage (m)/erythroblast (E)/megakaryocyte (M) (nmEM) colonies. Assuming that a substantial portion of long-term repopulating cells can be detected as nmEM cells within this population, we compared differentiation potentials between individual pairs of daughter and granddaughter cells derived in vitro from single nmEM cells. One of the two daughter or granddaughter cells remained an nmEM cell. The other showed a variety of combinations of differentiation potential. In particular, an nmEM cell directly gave rise, after one cell division, to progenitor cells committed to nm, EM, or M lineages. The probability of asymmetric division of nmEM cells depended on the cytokines used. These data strongly suggest that lineage commitment takes place asymmetrically at the level of HSCs under the influence of external factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.