ArticleThe Journal of experimental medicine2004
Insulin induces the release of vasodilator compounds from platelets by a nitric oxide-G kinase-VAMP-3-dependent pathway.
Article in The Journal of experimental medicine, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 67 citations in OpenAlex.
- Endothelial nitric oxide signaling in cellular and systemic metabolism.Redox biology · 2026Review
- Effects of vasopressin and antihyperkalemic treatments on hemodynamics, potassium concentration, and resuscitation success in a porcine hyperkalemic cardiac arrest model.Scientific reports · 2026Article
- Reciprocal tumor-platelet interaction through the EPHB1-EFNB1 axis in the liver metastatic niche promotes metastatic tumor outgrowth in pancreatic ductal adenocarcinoma.Cancer communications (London, England) · 2025Article
- Association between O-GlcNAc levels and platelet function in obese insulin-resistant subjects.Glycoconjugate journal · 2024Article
- Phosphoproteomic response of cardiac endothelial cells to ischemia and ultrasound.Biochimica et biophysica acta. Proteins and proteomics · 2021Article
- Cyclin Y is expressed in Platelets and Modulates Integrin Outside-in Signaling.International journal of molecular sciences · 2020Article
- Role of NO and S-nitrosylation in the Expression of Endothelial Adhesion Proteins That Regulate Leukocyte and Tumor Cell Adhesion.Frontiers in physiology · 2020Review
- Insulin Is Transcribed and Translated in Mammalian Taste Bud Cells.Endocrinology · 2018Article
- The eNOS signalosome and its link to endothelial dysfunction.Pflugers Archiv : European journal of physiology · 2016Review
- Overview of platelet physiology: its hemostatic and nonhemostatic role in disease pathogenesis.TheScientificWorldJournal · 2014Article
- Methylglyoxal induces platelet hyperaggregation and reduces thrombus stability by activating PKC and inhibiting PI3K/Akt pathway.PloS one · 2013Article
- Article
- Signaling during platelet adhesion and activation.Arteriosclerosis, thrombosis, and vascular biology · 2010Review
- Altered nitric oxide/cGMP platelet signaling pathway in platelets from patients with acute coronary syndromes.Clinical research in cardiology : official journal of the German Cardiac Society · 2010Article
- Molecular mechanisms underlying the activation of eNOS.Pflugers Archiv : European journal of physiology · 2010Review
- An important role of the SRC family kinase Lyn in stimulating platelet granule secretion.The Journal of biological chemistry · 2010Article
- Insulin, insulin resistance, and platelet signaling in diabetes.Diabetes care · 2009Article
- Nitric oxide regulation of protein trafficking in the cardiovascular system.Cardiovascular research · 2007Review
- Article
- Nitric oxide regulates endocytosis by S-nitrosylation of dynamin.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insulin-induced vasodilatation is sensitive to nitric oxide (NO) synthase (NOS) inhibitors. However, insulin is unable to relax isolated arteries or to activate endothelial NOS in endothelial cells. Since insulin can enhance platelet endothelial NOS activity, we determined whether insulin-induced vasodilatation can be attributed to a NO-dependent, platelet-mediated process. Insulin failed to relax endothelium-intact rings of porcine coronary artery. The supernatant from insulin-stimulated human platelets induced complete relaxation, which was prevented by preincubation of platelets with a NOS inhibitor, the soluble guanylyl cyclase inhibitor, NS 2028, or the G kinase inhibitor, KT 5823, and was abolished by an adenosine A2A receptor antagonist. Insulin induced the release of adenosine trisphosphate (ATP), adenosine, and serotonin from platelet-dense granules in a NO-dependent manner. This response was not detected using insulin-stimulated platelets from endothelial NOS-/- mice, although a NO donor elicited ATP release. Insulin-induced ATP release from human platelets correlated with the association of syntaxin 2 with the vesicle-associated membrane protein 3 but was not associated with the activation of alphaIIbbeta3 integrin. Thus, insulin elicits the release of vasoactive concentrations of ATP and adenosine from human platelets via a NO-G kinase-dependent signaling cascade. The mechanism of dense granule secretion involves the G kinase-dependent association of syntaxin 2 with vesicle-associated membrane protein 3.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.