ArticleThe Journal of general physiology2004
A store-operated calcium channel in Drosophila S2 cells.
Article in The Journal of general physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 80 citations in OpenAlex.
- Side-by-side comparison of published small molecule inhibitors against thapsigargin-induced store-operated Ca2+ entry in HEK293 cells.PloS one · 2024Article
- Neuronal Store-Operated Calcium Channels.Molecular neurobiology · 2023Review
- Regulatory mechanisms controlling store-operated calcium entry.Frontiers in physiology · 2023Review
- Mechanisms of ion selectivity and throughput in the mitochondrial calcium uniporter.Science advances · 2022Article
- Cell-wide mapping of Orai1 channel activity reveals functional heterogeneity in STIM1-Orai1 puncta.The Journal of general physiology · 2020Article
- STIM calcium sensing and conformational change.The Journal of physiology · 2020Review
- ROS regulation of axonal mitochondrial transport is mediated by Ca2+ and JNK in Drosophila.PloS one · 2017Article
- Pharmacological Characterization of the Native Store-Operated Calcium Channels of Cortical Neurons from Embryonic Mouse Brain.Frontiers in pharmacology · 2016Article
- The STIM1-ORAI1 microdomain.Cell calcium · 2015Review
- Store-Operated Calcium Channels.Physiological reviews · 2015Review
- Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS.Nature communications · 2015Article
- Store-operated calcium entry: Mechanisms and modulation.Biochemical and biophysical research communications · 2015Review
- Molecular biophysics of Orai store-operated Ca2+ channels.Biophysical journal · 2015Review
- State-dependent block of Orai3 TM1 and TM3 cysteine mutants: insights into 2-APB activation.The Journal of general physiology · 2014Article
- Differential roles of the C and N termini of Orai1 protein in interacting with stromal interaction molecule 1 (STIM1) for Ca2+ release-activated Ca2+ (CRAC) channel activation.The Journal of biological chemistry · 2013Article
- How many Orai's does it take to make a CRAC channel?Scientific reports · 2013Article
- Crystal structure of the calcium release-activated calcium channel Orai.Science (New York, N.Y.) · 2012Article
- Permeation, selectivity and gating in store-operated CRAC channels.The Journal of physiology · 2012Review
- Regulation of store-operated calcium entry during cell division.Biochemical Society transactions · 2012Article
- Subunit stoichiometry of human Orai1 and Orai3 channels in closed and open states.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
Using whole-cell recording in Drosophila S2 cells, we characterized a Ca(2+)-selective current that is activated by depletion of intracellular Ca2+ stores. Passive store depletion with a Ca(2+)-free pipette solution containing 12 mM BAPTA activated an inwardly rectifying Ca2+ current with a reversal potential >60 mV. Inward currents developed with a delay and reached a maximum of 20-50 pA at -110 mV. This current doubled in amplitude upon increasing external Ca2+ from 2 to 20 mM and was not affected by substitution of choline for Na+. A pipette solution containing approximately 300 nM free Ca2+ and 10 mM EGTA prevented spontaneous activation, but Ca2+ current activated promptly upon application of ionomycin or thapsigargin, or during dialysis with IP3. Isotonic substitution of 20 mM Ca2+ by test divalent cations revealed a selectivity sequence of Ba2+ > Sr2+ > Ca2+ >> Mg2+. Ba2+ and Sr2+ currents inactivated within seconds of exposure to zero-Ca2+ solution at a holding potential of 10 mV. Inactivation of Ba2+ and Sr2+ currents showed recovery during strong hyperpolarizing pulses. Noise analysis provided an estimate of unitary conductance values in 20 mM Ca2+ and Ba2+ of 36 and 420 fS, respectively. Upon removal of all external divalent ions, a transient monovalent current exhibited strong selectivity for Na+ over Cs+. The Ca2+ current was completely and reversibly blocked by Gd3+, with an IC50 value of approximately 50 nM, and was also blocked by 20 microM SKF 96365 and by 20 microM 2-APB. At concentrations between 5 and 14 microM, application of 2-APB increased the magnitude of Ca2+ currents. We conclude that S2 cells express store-operated Ca2+ channels with many of the same biophysical characteristics as CRAC channels in mammalian cells.
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