ArticleInvestigative ophthalmology & visual science2004
Osmoregulation of taurine transporter function and expression in retinal pigment epithelial, ganglion, and müller cells.
Article in Investigative ophthalmology & visual science, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 72 citations in OpenAlex.
- Taurine deficiency is a cause of vigabatrin-induced retinal phototoxicity.Annals of neurology · 2009Trial
- Clinical implications of taurine in breast cancer.Biochemistry and biophysics reports · 2025Review
- Myopia and Metabolomics: A Comparative Study of Aqueous Humor and Serum Metabolites in Myopic Adults Undergoing Cataract Surgery.International journal of molecular sciences · 2025Article
- Therapeutic Effects of Anti-Inflammatory and Anti-Oxidant Nutritional Supplementation in Retinal Ischemic Diseases.International journal of molecular sciences · 2024Review
- Vitamin A aldehyde-taurine adducts function in photoreceptor cells.Redox biology · 2022Article
- Dietary supplements in retinal diseases, glaucoma, and other ocular conditions.Journal of preventive medicine and hygiene · 2022Review
- Effects of Hydrostatic Pressure on Electrical Retinal Activity in a Multielectrode Array-BasedFrontiers in neuroscience · 2022Article
- Osmotic gradients and transretinal water flow-a quantitative elemental microanalytical study of frozen hydrated chick eyes.Frontiers in cellular neuroscience · 2022Article
- Taurine and oxidative stress in retinal health and disease.CNS neuroscience & therapeutics · 2021Review
- Detrimental Effects of UVB on Retinal Pigment Epithelial Cells and Its Role in Age-Related Macular Degeneration.Oxidative medicine and cellular longevity · 2020Review
- Roles of Drug Transporters in Blood-Retinal Barrier.Advances in experimental medicine and biology · 2019Review
- Potential Interplay between Hyperosmolarity and Inflammation on Retinal Pigmented Epithelium in Pathogenesis of Diabetic Retinopathy.International journal of molecular sciences · 2018Review
- Human retinal pigment epithelial cells prefer proline as a nutrient and transport metabolic intermediates to the retinal side.The Journal of biological chemistry · 2017Article
- Analysis of MTHFR, CBS, Glutathione, Taurine, and Hydrogen Sulfide Levels in Retinas of Hyperhomocysteinemic Mice.Investigative ophthalmology & visual science · 2017Article
- Involvement of TonEBP/NFAT5 in osmoadaptative response of human retinal pigmented epithelial cells to hyperosmolar stress.Molecular vision · 2016Article
- Origins and consequences of hyperosmolar stress in retinal pigmented epithelial cells.Frontiers in physiology · 2014Review
- A metabolomic approach to animal vitreous humor topographical composition: a pilot study.PloS one · 2014Article
- Hyperosmotic stress induces cell cycle arrest in retinal pigmented epithelial cells.Cell death & disease · 2013Article
- Decrease in hyperosmotic stress-induced corneal epithelial cell apoptosis by L-carnitine.Molecular vision · 2013Article
- Molecular and biochemical characterization of folate transport proteins in retinal Müller cells.Investigative ophthalmology & visual science · 2010Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo determine whether taurine transporter (TauT) activity and expression are regulated by hyperosmolarity in RPE, ganglion, and Müller cells.
methodsUptake of taurine was measured in ARPE-19 cells cultured in DMEM-F12 medium without or with the addition of 50 mM NaCl or 100 mM mannitol. The kinetics of the transport were analyzed. RT-PCR and Northern and Western blot analyses were used to assess TauT mRNA and protein levels. The influence of hyperosmolarity on the uptake of taurine, myo-inositol, and gamma-aminobutyric acid GABA was studied in RPE, RGC-5, and rMC1 cells.
resultsTauT activity was abundant in RPE and was stimulated (3.5-fold) when the cells were exposed to hyperosmolar conditions (DMEM-F12 culture medium plus 50 mM NaCl or 100 mM mannitol). Peak stimulation of taurine uptake occurred after 17 hours of exposure to hyperosmolar medium. Kinetic analysis revealed that the hyperosmolarity-induced stimulation was associated with an increase in V(max) of TauT with no change in K(m). TauT mRNA and protein levels increased in RPE cells exposed to hyperosmolar conditions. Hyperosmolarity also stimulated the uptake of myo-inositol ( approximately 15-fold); GABA uptake was influenced less markedly. Immunofluorescence and functional studies showed that TauT is present in cultured RGC-5 and rMC1 cells. TauT activity was robust in these cells in normal osmolar conditions and increased by approximately twofold in hyperosmolar conditions.
conclusionsThese studies provide the first evidence that hyperosmolarity regulates TauT activity and expression in RPE and that TauT is present in ganglion and Müller cells and is regulated by hypertonicity. The data are relevant to diseases such as diabetes, macular degeneration, and neurodegeneration, in which retinal cell volumes may fluctuate dramatically.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.