ArticleBiochemistry2004
Discrimination between different methylation states of chemotaxis receptor Tar by receptor methyltransferase CheR.
Article in Biochemistry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Article
- Methylation-Independent Chemotaxis Systems Are the Norm for Gastric-ColonizingJournal of bacteriology · 2022Article
- Sequential modification of bacterial chemoreceptors is key for achieving both accurate adaptation and high gain.Nature communications · 2020Article
- Methyltransferase CheR binds to its chemoreceptor substrates independent of their signaling conformation yet modifies them differentially.Protein science : a publication of the Protein Society · 2020Article
- Behavioral Variability and Phenotypic Diversity in Bacterial Chemotaxis.Annual review of biophysics · 2018Review
- Paradoxical enhancement of chemoreceptor detection sensitivity by a sensory adaptation enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Universal response-adaptation relation in bacterial chemotaxis.Journal of bacteriology · 2015Article
- An insight into the interaction mode between CheB and chemoreceptor from two crystal structures of CheB methylesterase catalytic domain.Biochemical and biophysical research communications · 2011Article
- The Pseudomonas aeruginosa chemotaxis methyltransferase CheR1 impacts on bacterial surface sampling.PloS one · 2011Article
- Molecular modeling of flexible arm-mediated interactions between bacterial chemoreceptors and their modification enzyme.Protein science : a publication of the Protein Society · 2009Article
- Evolutionary genomics reveals conserved structural determinants of signaling and adaptation in microbial chemoreceptors.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Characterization of the Thermotoga maritima chemotaxis methylation system that lacks pentapeptide-dependent methyltransferase CheR:MCP tethering.Molecular microbiology · 2007Article
- Identification of methylation sites in Thermotoga maritima chemotaxis receptors.Journal of bacteriology · 2006Article
- Site-specific and synergistic stimulation of methylation on the bacterial chemotaxis receptor Tsr by serine and CheW.BMC microbiology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
Bacterial chemotaxis receptors are posttranslationally modified by carboxyl methylation of specific glutamate residues within their cytoplasmic domains. This highly regulated, reversible modification counterbalances the signaling effects of ligand binding and contributes to adaptation. On the basis of the crystal structure of the gamma-glutamyl methyltransferase CheR, we have postulated that positively charged residues in helix alpha2 in the N-terminal domain of the enzyme may be complementary to the negatively charged methylation region of the methyltransferase substrates, the bacterial chemotaxis receptors. Several altered CheR proteins, in which positively charged arginine or lysine residues were substituted with alanines, were constructed and assayed for their methylation activities toward wild-type receptor and a series of receptor variants containing different glutamates available for methylation. One of the CheR mutant proteins (Arg53Ala) showed significantly lower activity toward all receptor constructs, suggesting that Arg53 may play a general role in catalysis of methyl transfer. The rest of the mutant proteins exhibited different patterns of relative methylation rates toward different receptor substrates, indicating specificity, probably through interaction of CheR with the receptor at sites distal to the specific site of methylation. The findings imply complementarity between positively charged residues of the alpha2 helix of CheR and the negatively charged glutamates of the receptor. It is likely that this complementarity is involved in discriminating different methylation states of the receptors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.