ArticleMolecular biology of the cell2004
Sti1 and Cdc37 can stabilize Hsp90 in chaperone complexes with a protein kinase.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 86 citations in OpenAlex.
- Insights into Hsp90 mechanism andFrontiers in molecular biosciences · 2024Review
- Characterization and regulation of salt upregulated cyclophilin from a halotolerant strain of Penicillium oxalicum.Scientific reports · 2023Article
- Article
- Detecting Posttranslational Modifications of Hsp90 Isoforms.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Comprehensive characterization of the Hsp70 interactome reveals novel client proteins and interactions mediated by posttranslational modifications.PLoS biology · 2022Article
- The Potential of Hsp90 in Targeting Pathological Pathways in Cardiac Diseases.Journal of personalized medicine · 2021Review
- General Structural and Functional Features of Molecular Chaperones.Advances in experimental medicine and biology · 2021Article
- Structure, Function, and Regulation of the Hsp90 Machinery.Cold Spring Harbor perspectives in biology · 2019Review
- Detecting Posttranslational Modifications of Hsp90.Methods in molecular biology (Clifton, N.J.) · 2018Article
- HSP90 supports tumor growth and angiogenesis through PRKD2 protein stabilization.Cancer research · 2014Article
- Mutation of essential Hsp90 co-chaperones SGT1 or CNS1 renders yeast hypersensitive to overexpression of other co-chaperones.Current genetics · 2014Article
- The ribosomal biogenesis protein Utp21 interacts with Hsp90 and has differing requirements for Hsp90-associated proteins.PloS one · 2014Article
- Contributions of co-chaperones and post-translational modifications towards Hsp90 drug sensitivity.Future medicinal chemistry · 2013Review
- Regulation of molecular chaperones through post-translational modifications: decrypting the chaperone code.Biochimica et biophysica acta · 2013Review
- Tumor-intrinsic and tumor-extrinsic factors impacting hsp90- targeted therapy.Current molecular medicine · 2012Review
- Chaperoning the chaperone: a role for the co-chaperone Cpr7 in modulating Hsp90 function in Saccharomyces cerevisiae.Genetics · 2012Article
- Quality control and fate determination of Hsp90 client proteins.Biochimica et biophysica acta · 2012Review
- Post-translational modifications of Hsp90 and their contributions to chaperone regulation.Biochimica et biophysica acta · 2012Review
- Detecting HSP90 phosphorylation.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Hsp110 chaperones control client fate determination in the hsp70-Hsp90 chaperone system.Molecular biology of the cell · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hsp90 functions in association with several cochaperones for folding of protein kinases and transcription factors, although the relative contribution of each to the overall reaction is unknown. We assayed the role of nine different cochaperones in the activation of Ste11, a Saccharomyces cerevisiae mitogen-activated protein kinase kinase kinase. Studies on signaling via this protein kinase pathway was measured by alpha-factor-stimulated induction of FIG1 or lacZ, and repression of HHF1. Several cochaperone mutants tested had reduced FIG1 induction or HHF1 repression, although to differing extents. The greatest defects were in cpr7Delta, sse1Delta, and ydj1Delta mutants. Assays of Ste11 kinase activity revealed a pattern of defects in the cochaperone mutant strains that were similar to the gene expression studies. Overexpression of CDC37, a chaperone required for protein kinase folding, suppressed defects the sti1Delta mutant back to wild-type levels. CDC37 overexpression also restored stable Hsp90 binding to the Ste11 protein kinase domain in the sti1Delta mutant strain. These data suggest that Cdc37 and Sti1 have functional overlap in stabilizing Hsp90:client complexes. Finally, we show that Cns1 functions in MAP kinase signaling in association with Cpr7.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.